دورية أكاديمية
An assay to measure poly(ADP ribose) glycohydrolase (PARG) activity in cells [version 2; referees: 2 approved]
| العنوان: | An assay to measure poly(ADP ribose) glycohydrolase (PARG) activity in cells [version 2; referees: 2 approved] |
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| المؤلفون: | Dominic I. James, Stephen Durant, Kay Eckersley, Emma Fairweather, Louise A. Griffiths, Nicola Hamilton, Paul Kelly, Mark O'Connor, Kerry Shea, Ian D. Waddell, Donald J. Ogilvie |
| المصدر: | F1000Research, Vol 5 (2016) |
| بيانات النشر: | F1000 Research Ltd, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Cell Signaling, Medical Genetics, Structure: Replication & Repair, Medicine, Science |
| الوصف: | After a DNA damage signal multiple polymers of ADP ribose attached to poly(ADP) ribose (PAR) polymerases (PARPs) are broken down by the enzyme poly(ADP) ribose glycohydrolase (PARG). Inhibition of PARG leads to a failure of DNA repair and small molecule inhibition of PARG has been a goal for many years. To determine whether biochemical inhibitors of PARG are active in cells we have designed an immunofluorescence assay to detect nuclear PAR after DNA damage. This 384-well assay is suitable for medium throughput high-content screening and can detect cell-permeable inhibitors of PARG from nM to µM potency. In addition, the assay has been shown to work in murine cells and in a variety of human cancer cells. Furthermore, the assay is suitable for detecting the DNA damage response induced by treatment with temozolomide and methylmethane sulfonate (MMS). Lastly, the assay has been shown to be robust over a period of several years. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2046-1402 |
| Relation: | http://f1000research.com/articles/5-736/v2; https://doaj.org/toc/2046-1402 |
| DOI: | 10.12688/f1000research.8463.2 |
| URL الوصول: | https://doaj.org/article/ba6ce5180d66401bab9aed77f463d36a |
| رقم الأكسشن: | edsdoj.ba6ce5180d66401bab9aed77f463d36a |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20461402 |
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| DOI: | 10.12688/f1000research.8463.2 |