دورية أكاديمية
Functional characterization of a farnesyl diphosphate synthase from Dendrobium nobile Lindl
| العنوان: | Functional characterization of a farnesyl diphosphate synthase from Dendrobium nobile Lindl |
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| المؤلفون: | Daoyong Gong, Bin Wu, Hongting Qin, Dezhao Fu, Shunxing Guo, Bochu Wang, Biao Li |
| المصدر: | AMB Express, Vol 12, Iss 1, Pp 1-12 (2022) |
| بيانات النشر: | SpringerOpen, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biotechnology LCC:Microbiology |
| مصطلحات موضوعية: | Dendrobium nobile Lindl., Farnesyl diphosphate synthase, Bioinformatics, Methyl jasmonate, Dendrobine, Biotechnology, TP248.13-248.65, Microbiology, QR1-502 |
| الوصف: | Abstract Dendrobium nobile Lindl. has been used as a traditional Chinese medicine for a long time, in which the most important compound is dendrobine functioning in a variety of pharmacological activities. Farnesyl diphosphate synthase (FPPS) is one of the key enzymes in the biosynthetic pathway of dendrobine. In this work, we found the expression profiles of DnFPPS were correlated with the contents of dendrobine under the methyl jasmonate (MeJA) treatments at different time. Then, the cloning and functional identification of a novel FPPS from D. nobile. The full length of DnFPPS is 1231 bp with an open reading frame of 1047 bp encoding 348 amino acids. The sequence similarity analysis demonstrated that DnFPPS was in the high homology with Dendrobium huoshanense and Dendrobium catenatum and contained four conserved domains. Phylogenetic analysis showed that DnFPPS was the close to the DhFPPS. Then, DnFPPS was induced to express in Escherichia coli, purified, and identified by SDS-PAGE electrophoresis. Gas chromatography-mass spectrometry analysis indicated that DnFPPS could catalyze dimethylallyl pyrophosphate and isopentenyl pyrophosphate to produce farnesyl diphosphate. Taken together, a novel DnFPPS was cloned and functionally identified, which supplied a candidate gene for the biosynthetic pathway of dendrobine. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2191-0855 |
| Relation: | https://doaj.org/toc/2191-0855 |
| DOI: | 10.1186/s13568-022-01470-2 |
| URL الوصول: | https://doaj.org/article/ba93409e2ce74f37bf1d5bd642a0933b |
| رقم الأكسشن: | edsdoj.ba93409e2ce74f37bf1d5bd642a0933b |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 21910855 |
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| DOI: | 10.1186/s13568-022-01470-2 |