دورية أكاديمية
Intron retention coupled with nonsense-mediated decay is involved in cellulase biosynthesis in cellulolytic fungi
| العنوان: | Intron retention coupled with nonsense-mediated decay is involved in cellulase biosynthesis in cellulolytic fungi |
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| المؤلفون: | Yichen Gao, Ai-Ping Pang, Leyao Ma, Haiyan Wang, Samran Durrani, Bingzhi Li, Fu-Gen Wu, Fengming Lin |
| المصدر: | Biotechnology for Biofuels and Bioproducts, Vol 15, Iss 1, Pp 1-13 (2022) |
| بيانات النشر: | BMC, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biotechnology LCC:Fuel |
| مصطلحات موضوعية: | Lignocellulose, Cellulase, Alternative splicing, Retained intron, NMD, Biotechnology, TP248.13-248.65, Fuel, TP315-360 |
| الوصف: | Abstract Background Knowledge on regulatory networks associated with cellulase biosynthesis is prerequisite for exploitation of such regulatory systems in enhancing cellulase production with low cost. The biological functions of intron retention (IR) and nonsense-mediated mRNA decay (NMD) in filamentous fungi is lack of study, let alone their roles in cellulase biosynthesis. Results We found that major cellulase genes (cel7a, cel7b, and cel3a) exhibited concomitant decrease in IR rates and increase in their gene expression in T. reesei under cellulase-producing condition (cellulose and lactose) that was accompanied with a more active NMD pathway, as compared to cellulase non-producing condition (glucose). In the presence of the NMD pathway inhibitor that successfully repressed the NMD pathway, the mRNA levels of cellulase genes were sharply down-regulated, but the rates of IR in these genes were significantly up-regulated. Consistently, the cellulase activities were severely inhibited. In addition, the NMD pathway inhibitor caused the downregulated mRNA levels of two important genes of the target of rapamycin (TOR) pathway, trfkbp12 and trTOR1. The absence of gene trfkbp12 made the cellulase production in T. reesei more sensitive to the NMD pathway inhibitor. Conclusions All these findings suggest that the IR of cellulase genes regulates their own gene expression by coupling with the NMD pathway, which might involve the TOR pathway. Our results provide better understanding on intron retention, the NMD pathway, and cellulase production mechanism in filamentous fungi. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2731-3654 |
| Relation: | https://doaj.org/toc/2731-3654 |
| DOI: | 10.1186/s13068-022-02141-x |
| URL الوصول: | https://doaj.org/article/baa241a00ca840778ba257148f17e6c0 |
| رقم الأكسشن: | edsdoj.baa241a00ca840778ba257148f17e6c0 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 27313654 |
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| DOI: | 10.1186/s13068-022-02141-x |