دورية أكاديمية
A novel and efficient murine model for investigating tendon-to-bone healing
| العنوان: | A novel and efficient murine model for investigating tendon-to-bone healing |
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| المؤلفون: | Baoyun Xu, Yunjiao Wang, Gang He, Kang-lai Tang, Lin Guo, Wan Chen |
| المصدر: | Journal of Orthopaedic Surgery and Research, Vol 19, Iss 1, Pp 1-10 (2024) |
| بيانات النشر: | BMC, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Orthopedic surgery LCC:Diseases of the musculoskeletal system |
| مصطلحات موضوعية: | Tendon-to-bone healing, Mouse animal model, The Achilles tendon, Enthesis, Orthopedic surgery, RD701-811, Diseases of the musculoskeletal system, RC925-935 |
| الوصف: | Abstract Background Tendon-to-bone healing is a critical challenge in sports medicine, with its cellular and molecular mechanisms yet to be explored. An efficient murine model could significantly advance our understanding of this process. However, most existing murine animal models face limitations, including a propensity for bleeding, restricted operational space, and a steep learning curve. Thus, the need for a novel and efficient murine animal model to investigate the cellular and molecular mechanisms of tendon-to-bone healing is becoming increasingly evident. Methods In our study, forty-four 9-week-old male C57/BL6 mice underwent transection and reattachment of the Achilles tendon insertion to investigate tendon-to-bone healing. At 2 and 4 weeks postoperatively, mice were killed for histological, Micro-CT, biomechanical, and real-time polymerase chain reaction tests. Results Histological staining revealed that the original tissue structure was disrupted and replaced by a fibrovascular scar. Although glycosaminoglycan deposition was present in the cartilage area, the native structure had been destroyed. Biomechanical tests showed that the failure force constituted approximately 44.2% and 77.5% of that in intact tissues, and the ultimate tensile strength increased from 2 to 4 weeks postoperatively. Micro-CT imaging demonstrated a gradual healing process in the bone tunnel from 2 to 4 weeks postoperatively. The expression levels of ACAN, SOX9, Collagen I, and MMPs were detected, with all genes being overexpressed compared to the control group and maintaining high levels at 2 and 4 weeks postoperatively. Conclusions Our results demonstrate that the healing process in our model is aligned with the natural healing process, suggesting the potential for creating a new, efficient, and reproducible mouse animal model to investigate the cellular and molecular mechanisms of tendon-to-bone healing. Graphical abstract |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1749-799X 69356815 |
| Relation: | https://doaj.org/toc/1749-799X |
| DOI: | 10.1186/s13018-023-04496-9 |
| URL الوصول: | https://doaj.org/article/ebaf46b67cce45c3b99a69356815cdbd |
| رقم الأكسشن: | edsdoj.baf46b67cce45c3b99a69356815cdbd |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 1749799X 69356815 |
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| DOI: | 10.1186/s13018-023-04496-9 |