دورية أكاديمية
Salvianolic acid A promotes mitochondrial biogenesis and mitochondrial function in 3T3-L1 adipocytes through regulation of the AMPK-PGC1α signalling pathway
| العنوان: | Salvianolic acid A promotes mitochondrial biogenesis and mitochondrial function in 3T3-L1 adipocytes through regulation of the AMPK-PGC1α signalling pathway |
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| المؤلفون: | Jialin Sun, Ping Leng, Xiao Li, Qie Guo, Jun Zhao, Yu Liang, Xiaolei Zhang, Xue Yang, Jing Li |
| المصدر: | Adipocyte, Vol 11, Iss 1, Pp 562-571 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Diseases of the endocrine glands. Clinical endocrinology LCC:Cytology LCC:Physiology |
| مصطلحات موضوعية: | Salvianolic acid A, 3T3-L1 adipocytes, mitochondrial biogenesis, mitochondrial function, AMPK, PGC-1α, Diseases of the endocrine glands. Clinical endocrinology, RC648-665, Cytology, QH573-671, Physiology, QP1-981 |
| الوصف: | Mitochondrial dysfunction is associated with insulin resistance and type 2 diabetes (T2DM). Decreased mitochondrial abundance and function were found in white adipose tissue (WAT) of T2DM patients. Therefore, promoting WAT mitochondrial biogenesis and improving adipocyte metabolism may be strategies to prevent and reverse T2DM. Salvianolic acid A (SAA) has been found to exert anti-diabetic and lipid disorder-improving effects. However whether SAA benefits mitochondrial biogenesis and function in adipose tissue is unclear. Here, we evaluated SAA’s effect on mitochondrial biogenesis and function in 3T3-L1 adipocytes and investigated its potential regulatory mechanism. Results showed that SAA treatment significantly promoted the transcription and expression of peroxisome proliferator-activated receptor γ coactivator- 1α (PGC-1α), nuclear respiratory factor 1 (NRF1) and mitochondrial transcription factor A (TFAM). Meanwhile, SAA treatment significantly promoted mitochondrial biogenesis by increasing mitochondrial DNA (mtDNA) quantity, mitochondrial mass, and expression of mitochondrial respiratory chain enzyme complexes III and complex IV. These enhancements were accompanied by enhanced phosphorylation of AMPK and ACC and were suppressed by Compound C, a specific AMPK inhibitor. Furthermore, SAA treatment improved adipocytes mitochondrial respiration and stimulated ATP generation. These findings indicate that SAA exerts a potential therapeutic capacity against adipocytes mitochondrial dysfunction in diabetes by activating the AMPK-PGC-1α pathway. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 21623945 2162-397X 2162-3945 |
| Relation: | https://doaj.org/toc/2162-3945; https://doaj.org/toc/2162-397X |
| DOI: | 10.1080/21623945.2022.2116790 |
| URL الوصول: | https://doaj.org/article/bb05d32e185f41a99d5655b2835b2b75 |
| رقم الأكسشن: | edsdoj.bb05d32e185f41a99d5655b2835b2b75 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 21623945 2162397X |
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| DOI: | 10.1080/21623945.2022.2116790 |