دورية أكاديمية
The Screening of Broadly Neutralizing Antibodies Targeting the SARS-CoV-2 Spike Protein by mRNA Immunization in Mice
| العنوان: | The Screening of Broadly Neutralizing Antibodies Targeting the SARS-CoV-2 Spike Protein by mRNA Immunization in Mice |
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| المؤلفون: | Zhiyin An, Yu Zhang, Xiang Yu, Jia Xia, Yanan Yin, Guoming Li, Jing Lu, Xuemei Fan, Yingjie Xu |
| المصدر: | Pharmaceutics, Vol 15, Iss 5, p 1412 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Pharmacy and materia medica |
| مصطلحات موضوعية: | SARS-CoV-2, spike, mRNA vaccine, broadly neutralizing antibody, Pharmacy and materia medica, RS1-441 |
| الوصف: | Neutralizing antibodies (nAbs), the popular antiviral drugs used for the treatment of COVID-19, are effective in reducing viral load and hospitalization. Currently, most nAbs are screened from convalescent or vaccinated individuals through single B-cell sequencing which requires cutting-edge facilities. Moreover, owing to the rapid mutation of SARS-CoV-2, some approved nAbs are no longer effective. In the present study, we designed a new approach to acquiring broadly neutralizing antibodies (bnAbs) from mRNA-vaccinated mice. Using the flexibility and speed of mRNA vaccine preparation, we designed a chimeric mRNA vaccine and sequential immunization strategies to acquire bnAbs in mice within a short period. By comparing different vaccination orders, we found that the initially administered vaccine had a greater effect on the neutralizing potency of mouse sera. Ultimately, we screened a strain of bnAb that neutralized wild-type, Beta, and Delta SARS-CoV-2 pseudoviruses. We synthesized the mRNAs of the heavy and light chains of this antibody and verified its neutralizing potency. This study developed a new strategy to screen for bnAbs in mRNA-vaccinated mice and identified a more effective immunization strategy for inducing bnAbs, providing valuable insights for future antibody drug development. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 15051412 1999-4923 |
| Relation: | https://www.mdpi.com/1999-4923/15/5/1412; https://doaj.org/toc/1999-4923 |
| DOI: | 10.3390/pharmaceutics15051412 |
| URL الوصول: | https://doaj.org/article/cbb1ba20a8c548ffb8508ddf3e8361ee |
| رقم الأكسشن: | edsdoj.bb1ba20a8c548ffb8508ddf3e8361ee |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 15051412 19994923 |
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| DOI: | 10.3390/pharmaceutics15051412 |