دورية أكاديمية
hVEGF Increases Survival of Transplanted Hepatocytes Within Portal Radicles: Suggested Mechanism for Early Cell Engraftment
| العنوان: | hVEGF Increases Survival of Transplanted Hepatocytes Within Portal Radicles: Suggested Mechanism for Early Cell Engraftment |
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| المؤلفون: | Hagit Shani-Peretz, Vladislav Tsiperson, Gideon Shoshani, Ella Veitzman, Gera Neufeld, Yaacov Baruch |
| المصدر: | Cell Transplantation, Vol 14 (2005) |
| بيانات النشر: | SAGE Publishing, 2005. |
| سنة النشر: | 2005 |
| المجموعة: | LCC:Medicine |
| مصطلحات موضوعية: | Medicine |
| الوصف: | VEGF is a potent angiogenic factor that promotes hepatocyte growth, increases permeability of blood vessels, and induces vasodilatation, and may accelerate engraftment and function of transplanted hepatocytes. The aim was to study the effect of VEGF on early hepatocyte engraftment. Thirty-two Lewis syngeneic female rats underwent 70% partial hepatectomy. Eighteen received 240 ng VEGF 165 and 14 received saline for control. Thereafter, intrasplenic transplantation of 107 male hepatocytes was done. Semiquantitative analysis of PCR product of the SRY region of the Y-chromosome was performed. Paraffin-embedded sections were stained for H&E and for PCNA immunostaining. By PCR, male hepatocytes were identified in 8 livers out of 14 VEGF-treated rats at 24 – 48 h, compared with only 1 liver out of 8 controls. Transplanted cells were seen within portal vessels radicles in 7 out of 14 VEGF-treated rats for as long as 48 h posttransplantation, compared with only one control liver at 24 h. There was no histological sign of cell injury to transplanted or adjacent cells. Two weeks after transplantation male transplanted cells were identified in two out of four rats treated with hVEGF 165 and in one out of six rats treated with saline. No transplanted cells were detected within portal tracts 14 days after transplantation. hVEGF 165 enhances the presence of transplanted hepatocytes within portal vessels after transplantation. We suggest an additional mechanism for cell engraftment, whereby transplanted hepatocytes first stick to each other in the portal radicles. Later they become included in the liver parenchyma as groups of organized cells in a process stimulated by VEGF. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 0963-6897 1555-3892 00000000 |
| Relation: | https://doaj.org/toc/0963-6897; https://doaj.org/toc/1555-3892 |
| DOI: | 10.3727/000000005783983331 |
| URL الوصول: | https://doaj.org/article/cebb4411b87c4f70a362d7b3d9933285 |
| رقم الأكسشن: | edsdoj.bb4411b87c4f70a362d7b3d9933285 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 09636897 15553892 00000000 |
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| DOI: | 10.3727/000000005783983331 |