دورية أكاديمية
miR-458b-5p regulates ovarian granulosa cells proliferation through Wnt/β-catenin signaling pathway by targeting catenin beta-1
| العنوان: | miR-458b-5p regulates ovarian granulosa cells proliferation through Wnt/β-catenin signaling pathway by targeting catenin beta-1 |
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| المؤلفون: | Wenwen Wang, Jun Teng, Xu Han, Shen Zhang, Qin Zhang, Hui Tang |
| المصدر: | Animal Bioscience, Vol 34, Iss 6, Pp 957-966 (2021) |
| بيانات النشر: | Asian-Australasian Association of Animal Production Societies, 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Zoology |
| مصطلحات موضوعية: | mir-458b-5p, catenin beta-1 (), granulosa cells, proliferation, wnt, β-catenin signaling pathway, Zoology, QL1-991 |
| الوصف: | Objective Ovarian follicular development, which dependent on the proliferation and differentiation of granulosa cells (GCs), is a complex biological process in which miRNA plays an important role. Our previous study showed that miR-458b-5p is associated with ovarian follicular development in chicken. The detailed function and molecular mechanism of miR-458b-5p in GCs is unclear. Methods The luciferase reporter assay was used to verify the targeting relationship between miR-458b-5p and catenin beta-1 (CTNNB1), which is an important transcriptional regulatory factor of the Wnt/β-catenin pathway. The cell counting kit-8 (CCK-8) assay, flow cytometry with propidium iodide (PI) and annexin V-fluorescein isothiocyanate (FITC) labeling were applied to explore the effect of miR-458b-5p on proliferation, cell cycle and apoptosis of chicken GCs. Quantitative real-time polymerase chain reaction and Western blot were used to detect the mRNA and protein expression levels. Results We demonstrated that the expression of miR-458b-5p and CTNNB1 showed the opposite relationship in GCs and theca cells of hierarchical follicles. The luciferase reporter assay confirmed that CTNNB1 is the direct target of miR-458b-5p. Using CCK-8 assay and flow cytometry with PI and Annexin V-FITC labeling, we observed that transfection with the miR-458b-5p mimics significantly reduced proliferation and has no effects on apoptosis of chicken GCs. In addition, miR-458b-5p decreased the mRNA and protein expression of CD44 molecule and matrix metallopeptidase 7, which are the downstream effectors of CTNNB1 in Wnt/β-Catenin pathway and play functional roles in cell proliferation. Conclusion Taken together, the data indicate that miR-458b-5p regulates ovarian GCs proliferation through Wnt/β-catenin signaling pathway by targeting CTNNB1, suggesting that miR-458b-5p and its target gene CTNNB1 may potentially play a role in chicken ovarian follicular development. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2765-0189 2765-0235 |
| Relation: | http://www.animbiosci.org/upload/pdf/ajas-20-0392.pdf; https://doaj.org/toc/2765-0189; https://doaj.org/toc/2765-0235 |
| DOI: | 10.5713/ajas.20.0392 |
| URL الوصول: | https://doaj.org/article/bb8fa9c8a99147d3a8587cd1a59d1c75 |
| رقم الأكسشن: | edsdoj.bb8fa9c8a99147d3a8587cd1a59d1c75 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 27650189 27650235 |
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| DOI: | 10.5713/ajas.20.0392 |