دورية أكاديمية
Transcriptional profiling at whole population and single cell levels reveals somatosensory neuron molecular diversity
| العنوان: | Transcriptional profiling at whole population and single cell levels reveals somatosensory neuron molecular diversity |
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| المؤلفون: | Isaac M Chiu, Lee B Barrett, Erika K Williams, David E Strochlic, Seungkyu Lee, Andy D Weyer, Shan Lou, Gregory S Bryman, David P Roberson, Nader Ghasemlou, Cara Piccoli, Ezgi Ahat, Victor Wang, Enrique J Cobos, Cheryl L Stucky, Qiufu Ma, Stephen D Liberles, Clifford J Woolf |
| المصدر: | eLife, Vol 3 (2014) |
| بيانات النشر: | eLife Sciences Publications Ltd, 2014. |
| سنة النشر: | 2014 |
| المجموعة: | LCC:Medicine LCC:Science LCC:Biology (General) |
| مصطلحات موضوعية: | transcriptome, peripheral nervous system, somatosensation, DRG, nociception, proprioception, Medicine, Science, Biology (General), QH301-705.5 |
| الوصف: | The somatosensory nervous system is critical for the organism's ability to respond to mechanical, thermal, and nociceptive stimuli. Somatosensory neurons are functionally and anatomically diverse but their molecular profiles are not well-defined. Here, we used transcriptional profiling to analyze the detailed molecular signatures of dorsal root ganglion (DRG) sensory neurons. We used two mouse reporter lines and surface IB4 labeling to purify three major non-overlapping classes of neurons: 1) IB4+SNS-Cre/TdTomato+, 2) IB4−SNS-Cre/TdTomato+, and 3) Parv-Cre/TdTomato+ cells, encompassing the majority of nociceptive, pruriceptive, and proprioceptive neurons. These neurons displayed distinct expression patterns of ion channels, transcription factors, and GPCRs. Highly parallel qRT-PCR analysis of 334 single neurons selected by membership of the three populations demonstrated further diversity, with unbiased clustering analysis identifying six distinct subgroups. These data significantly increase our knowledge of the molecular identities of known DRG populations and uncover potentially novel subsets, revealing the complexity and diversity of those neurons underlying somatosensation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2050-084X |
| Relation: | https://elifesciences.org/articles/04660; https://doaj.org/toc/2050-084X |
| DOI: | 10.7554/eLife.04660 |
| URL الوصول: | https://doaj.org/article/bc65ad0efece430bb716d97cb8616581 |
| رقم الأكسشن: | edsdoj.bc65ad0efece430bb716d97cb8616581 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2050084X |
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| DOI: | 10.7554/eLife.04660 |