دورية أكاديمية

AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.

التفاصيل البيبلوغرافية
العنوان: AKTIP interacts with ESCRT I and is needed for the recruitment of ESCRT III subunits to the midbody.
المؤلفون: Chiara Merigliano, Romina Burla, Mattia La Torre, Simona Del Giudice, Hsiangling Teo, Chong Wai Liew, Alexandre Chojnowski, Wah Ing Goh, Yolanda Olmos, Klizia Maccaroni, Maria Giubettini, Irene Chiolo, Jeremy G Carlton, Domenico Raimondo, Fiammetta Vernì, Colin L Stewart, Daniela Rhodes, Graham D Wright, Brian E Burke, Isabella Saggio
المصدر: PLoS Genetics, Vol 17, Iss 8, p e1009757 (2021)
بيانات النشر: Public Library of Science (PLoS), 2021.
سنة النشر: 2021
المجموعة: LCC:Genetics
مصطلحات موضوعية: Genetics, QH426-470
الوصف: To complete mitosis, the bridge that links the two daughter cells needs to be cleaved. This step is carried out by the endosomal sorting complex required for transport (ESCRT) machinery. AKTIP, a protein discovered to be associated with telomeres and the nuclear membrane in interphase cells, shares sequence similarities with the ESCRT I component TSG101. Here we present evidence that during mitosis AKTIP is part of the ESCRT machinery at the midbody. AKTIP interacts with the ESCRT I subunit VPS28 and forms a circular supra-structure at the midbody, in close proximity with TSG101 and VPS28 and adjacent to the members of the ESCRT III module CHMP2A, CHMP4B and IST1. Mechanistically, the recruitment of AKTIP is dependent on MKLP1 and independent of CEP55. AKTIP and TSG101 are needed together for the recruitment of the ESCRT III subunit CHMP4B and in parallel for the recruitment of IST1. Alone, the reduction of AKTIP impinges on IST1 and causes multinucleation. Our data altogether reveal that AKTIP is a component of the ESCRT I module and functions in the recruitment of ESCRT III components required for abscission.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1553-7390
1553-7404
Relation: https://doaj.org/toc/1553-7390; https://doaj.org/toc/1553-7404
DOI: 10.1371/journal.pgen.1009757
URL الوصول: https://doaj.org/article/bc66149e25c047faa2127e3e143af22e
رقم الأكسشن: edsdoj.bc66149e25c047faa2127e3e143af22e
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:15537390
15537404
DOI:10.1371/journal.pgen.1009757