دورية أكاديمية
أعرض في EDS

MicroRNA and mRNA signatures in ischemia reperfusion injury in heart transplantation.

التفاصيل البيبلوغرافية
العنوان: MicroRNA and mRNA signatures in ischemia reperfusion injury in heart transplantation.
المؤلفون: Liangyi Zhou, Guoyao Zang, Guangfeng Zhang, Hansong Wang, Xusheng Zhang, Nathan Johnston, Weiping Min, Patrick Luke, Anthony Jevnikar, Aaron Haig, Xiufen Zheng
المصدر: PLoS ONE, Vol 8, Iss 11, p e79805 (2013)
بيانات النشر: Public Library of Science (PLoS), 2013.
سنة النشر: 2013
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Ischemia reperfusion (I/R) injury is an unavoidable event occurring during heart transplantation, leading to graft failures and lower long-term survival rate of the recipient. Several studies have demonstrated that microRNAs (miRNAs) are vital regulators of signalling pathways involved in I/R injury. The present study aims to quantify the altered expression levels of miRNA and mRNA upon I/R injury in a mouse heart transplantation model, and to investigate whether these miRNA can regulate genes involved in I/R injury. We performed heterotopic heart transplantation on mouse models to generate heart tissue samples with I/R and non-I/R (control). The expression levels of miRNAs as well as genes were measured in heart grafts by microarray and real time RT-PCR. miRNA alteration in cardiomyocytes exposed to hypoxia was also detected by qRT-PCR. We observed significant alterations in miRNA and gene expression profile after I/R injury. There were 39 miRNAs significantly downregulated and 20 upregulated up to 1.5 fold in heart grafts with I/R injury compared with the grafts without I/R. 48 genes were observed with 3 fold change and p
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3835872?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0079805
URL الوصول: https://doaj.org/article/bd771e0cbf674a3fa25c205cea535b34
رقم الأكسشن: edsdoj.bd771e0cbf674a3fa25c205cea535b34
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0079805