دورية أكاديمية
Transcriptional Targeting of Dendritic Cells Using an Optimized Human Fascin1 Gene Promoter
| العنوان: | Transcriptional Targeting of Dendritic Cells Using an Optimized Human Fascin1 Gene Promoter |
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| المؤلفون: | Yanira Zeyn, Dominika Hobernik, Ulrich Wilk, Jana Pöhmerer, Christoph Hieber, Carolina Medina-Montano, Nadine Röhrig, Caroline F. Strähle, Andrea K. Thoma-Kress, Ernst Wagner, Matthias Bros, Simone Berger |
| المصدر: | International Journal of Molecular Sciences, Vol 24, Iss 23, p 16938 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | fascin1, cytomegalovirus, promotor activity, plasmid DNA, gene delivery, polyplex, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | Deeper knowledge about the role of the tumor microenvironment (TME) in cancer development and progression has resulted in new strategies such as gene-based cancer immunotherapy. Whereas some approaches focus on the expression of tumoricidal genes within the TME, DNA-based vaccines are intended to be expressed in antigen-presenting cells (e.g., dendritic cells, DCs) in secondary lymphoid organs, which in turn induce anti-tumor T cell responses. Besides effective delivery systems and the requirement of appropriate adjuvants, DNA vaccines themselves need to be optimized regarding efficacy and selectivity. In this work, the concept of DC-focused transcriptional targeting was tested by applying a plasmid encoding for the luciferase reporter gene under the control of a derivative of the human fascin1 gene promoter (pFscnLuc), comprising the proximal core promoter fused to the normally more distantly located DC enhancer region. DC-focused activity of this reporter construct was confirmed in cell culture in comparison to a standard reporter vector encoding for luciferase under the control of the strong ubiquitously active cytomegalovirus promoter and enhancer (pCMVLuc). Both plasmids were also compared upon intravenous administration in mice. The organ- and cell type-specific expression profile of pFscnLuc versus pCMVLuc demonstrated favorable activity especially in the spleen as a central immune organ and within the spleen in DCs. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/24/23/16938; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms242316938 |
| URL الوصول: | https://doaj.org/article/bd7ceeae1cc8487cb44e56bfe6e58dc7 |
| رقم الأكسشن: | edsdoj.bd7ceeae1cc8487cb44e56bfe6e58dc7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14220067 16616596 |
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| DOI: | 10.3390/ijms242316938 |