دورية أكاديمية
DNA and RNA Stability of Marine Microalgae in Cold-Stored Sediments and Its Implications in Metabarcoding Analyses
| العنوان: | DNA and RNA Stability of Marine Microalgae in Cold-Stored Sediments and Its Implications in Metabarcoding Analyses |
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| المؤلفون: | Zhaoyang Chai, Yuyang Liu, Siyang Jia, Fengting Li, Zhangxi Hu, Yunyan Deng, Caixia Yue, Ying-Zhong Tang |
| المصدر: | International Journal of Molecular Sciences, Vol 25, Iss 3, p 1724 (2024) |
| بيانات النشر: | MDPI AG, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Biology (General) LCC:Chemistry |
| مصطلحات موضوعية: | microalgae, DNA/RNA stability, resting cysts, sediments, metabarcoding, quantitative real-time PCR, Biology (General), QH301-705.5, Chemistry, QD1-999 |
| الوصف: | The ever-increasing applications of metabarcoding analyses for environmental samples demand a well-designed assessment of the stability of DNA and RNA contained in cells that are deposited or buried in marine sediments. We thus conducted a qPCR quantification of the DNA and RNA in the vegetative cells of three microalgae entrapped in facsimile marine sediments and found that >90% of DNA and up to 99% of RNA for all microalgal species were degraded within 60 days at 4 °C. A further examination of the potential interference of the relic DNA of the vegetative cells with resting cyst detection in sediments was performed via a metabarcoding analysis in artificial marine sediments spiked with the vegetative cells of two Kareniaceae dinoflagellates and the resting cysts of another three dinoflagellates. The results demonstrated a dramatic decrease in the relative abundances of the two Kareniaceae dinoflagellates in 120 days, while those of the three resting cysts increased dramatically. Together, our results suggest that a positive detection of microalgae via metabarcoding analysis in DNA or RNA extracted from marine sediments strongly indicates the presence of intact or viable cysts or spores due to the rapid decay of relic DNA/RNA. This study provides a solid basis for the data interpretation of metabarcoding surveys, particularly in resting cyst detection. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 25031724 1422-0067 1661-6596 |
| Relation: | https://www.mdpi.com/1422-0067/25/3/1724; https://doaj.org/toc/1661-6596; https://doaj.org/toc/1422-0067 |
| DOI: | 10.3390/ijms25031724 |
| URL الوصول: | https://doaj.org/article/bdbc271cf9d949bfb89aaaac7696eec0 |
| رقم الأكسشن: | edsdoj.bdbc271cf9d949bfb89aaaac7696eec0 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 25031724 14220067 16616596 |
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| DOI: | 10.3390/ijms25031724 |