دورية أكاديمية
Recognition of Single Fluorescence Events by Temporal Pixel Intensity Fluctuation
| العنوان: | Recognition of Single Fluorescence Events by Temporal Pixel Intensity Fluctuation |
|---|---|
| المؤلفون: | Kai Gu, Chunming Liu |
| المصدر: | Chemical & Biomedical Imaging, Vol 1, Iss 3, Pp 234-241 (2023) |
| بيانات النشر: | American Chemical Society, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Medical technology LCC:Chemistry |
| مصطلحات موضوعية: | Medical technology, R855-855.5, Chemistry, QD1-999 |
| الوصف: | Single-molecule localization microscopy circumvents the diffraction limit of traditional fluorescence microscopy by detecting the photoemission signals of individual fluorescent molecules. The accurate recognitions of fluorescence molecules/events are critical to single-molecule/super-resolution imaging experiments, which determine the precision of molecular localizations and the quality of the image reconstruction. Herein, we presented a single-molecule detection method which relied on the temporal pixel intensity fluctuation. The method was capable of quickly determining the approximate localizations of fluorescence events with high sensitivity. We evaluated the performance of the method under a series of signal-to-noise ratios (SNR) and discussed the criterion of setting the temporal fluctuation threshold to achieve the optimized spots recognition results. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2832-3637 |
| Relation: | https://doaj.org/toc/2832-3637 |
| DOI: | 10.1021/cbmi.3c00043 |
| URL الوصول: | https://doaj.org/article/bdc4d5fa4b1b4f4ca0467aa8c6024027 |
| رقم الأكسشن: | edsdoj.bdc4d5fa4b1b4f4ca0467aa8c6024027 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this issue from the American Chemical Society 
Full Text Finder | تدمد: | 28323637 |
|---|---|
| DOI: | 10.1021/cbmi.3c00043 |