دورية أكاديمية
Comparison of Next-Generation Sequencing and Polymerase Chain Reaction for Personalized Treatment-Related Genomic Status in Patients with Metastatic Colorectal Cancer
| العنوان: | Comparison of Next-Generation Sequencing and Polymerase Chain Reaction for Personalized Treatment-Related Genomic Status in Patients with Metastatic Colorectal Cancer |
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| المؤلفون: | Wei-Chih Su, Yi-Chen Tsai, Hsiang-Lin Tsai, Tsung-Kun Chang, Tzu-Chieh Yin, Ching-Wen Huang, Yen-Cheng Chen, Ching-Chun Li, Po-Jung Chen, Yun-Ru Liu, Tsung-Han Hsieh, Jaw-Yuan Wang |
| المصدر: | Current Issues in Molecular Biology, Vol 44, Iss 4, Pp 1552-1563 (2022) |
| بيانات النشر: | MDPI AG, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | next-generation sequencing, polymerase chain reaction, metastatic colorectal cancer, Biology (General), QH301-705.5 |
| الوصف: | Personalized treatments based on the genetic profiles of tumors can simultaneously optimize efficacy and minimize toxicity, which is beneficial for improving patient outcomes. This study aimed to integrate gene alterations associated with predictive and prognostic outcomes in patients with metastatic colorectal cancer (mCRC) with polymerase chain reaction (PCR) and in-house next-generation sequencing (NGS) to detect KRAS, NRAS, and BRAF mutations. In the present study, 41 patients with mCRC were assessed between August 2017 and June 2019 at a single institution. The overall concordance between NGS and PCR results for detecting KRAS, NRAS, and BRAF mutations was considerably high (87.8–92.7%), with only 15 discrepant results between PCR and NGS. Our companion diagnostic test analyzes KRAS, NRAS, and BRAF as a panel of CRC molecular targets; therefore, it has the advantages of requiring fewer specimens and being more time and cost efficient than conventional testing for separate analyses, allowing for the simultaneous analysis of multiple genes. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1467-3045 1467-3037 |
| Relation: | https://www.mdpi.com/1467-3045/44/4/106; https://doaj.org/toc/1467-3037; https://doaj.org/toc/1467-3045 |
| DOI: | 10.3390/cimb44040106 |
| URL الوصول: | https://doaj.org/article/be391802ddaa4c7b806bb867d711857e |
| رقم الأكسشن: | edsdoj.be391802ddaa4c7b806bb867d711857e |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 14673045 14673037 |
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| DOI: | 10.3390/cimb44040106 |