دورية أكاديمية
Concordance in diabetic foot ulceration: a cross-sectional study of agreement between wound swabbing and tissue sampling in infected ulcers
| العنوان: | Concordance in diabetic foot ulceration: a cross-sectional study of agreement between wound swabbing and tissue sampling in infected ulcers |
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| المؤلفون: | E Andrea Nelson, Alexandra Wright-Hughes, Sarah Brown, Benjamin A Lipsky, Michael Backhouse, Moninder Bhogal, Mwidimi Ndosi, Catherine Reynolds, Gill Sykes, Christopher Dowson, Michael Edmonds, Peter Vowden, Edward B Jude, Tom Dickie, Jane Nixon |
| المصدر: | Health Technology Assessment, Vol 20, Iss 82 (2016) |
| بيانات النشر: | NIHR Journals Library, 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Medical technology |
| مصطلحات موضوعية: | diabetic foot ulcer, infection, diagnostic agreement, microbiology techniques, prognosis, tissue sampling, swabbing, antibiotic therapy, polymerase chain reaction, Medical technology, R855-855.5 |
| الوصف: | Background: There is inadequate evidence to advise clinicians on the relative merits of swabbing versus tissue sampling of infected diabetic foot ulcers (DFUs). Objectives: To determine (1) concordance between culture results from wound swabs and tissue samples from the same ulcer; (2) whether or not differences in bacterial profiles from swabs and tissue samples are clinically relevant; (3) concordance between results from conventional culture versus polymerase chain reaction (PCR); and (4) prognosis for patients with an infected DFU at 12 months’ follow-up. Methods: This was a cross-sectional, multicentre study involving patients with diabetes and a foot ulcer that was deemed to be infected by their clinician. Microbiology specimens for culture were taken contemporaneously by swab and by tissue sampling from the same wound. In a substudy, specimens were also processed by PCR. A virtual ‘blinded’ clinical review compared the appropriateness of patients’ initial antibiotic regimens based on the results of swab and tissue specimens. Patients’ case notes were reviewed at 12 months to assess prognosis. Results: The main study recruited 400 patients, with 247 patients in the clinical review. There were 12 patients in the PCR study and 299 patients in the prognosis study. Patients’ median age was 63 years (range 26–99 years), their diabetes duration was 15 years (range 2 weeks–57 years), and their index ulcer duration was 1.8 months (range 3 days–12 years). Half of the ulcers were neuropathic and the remainder were ischaemic/neuroischaemic. Tissue results reported more than one pathogen in significantly more specimens than swabs {86.1% vs. 70.1% of patients, 15.9% difference [95% confidence interval (CI) 11.8% to 20.1%], McNemar’s p-value |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1366-5278 2046-4924 |
| Relation: | https://doaj.org/toc/1366-5278; https://doaj.org/toc/2046-4924 |
| DOI: | 10.3310/hta20820 |
| URL الوصول: | https://doaj.org/article/bea2609cb6214c3f89e44282ce48547b |
| رقم الأكسشن: | edsdoj.bea2609cb6214c3f89e44282ce48547b |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 13665278 20464924 |
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| DOI: | 10.3310/hta20820 |