دورية أكاديمية
BIITE: A Tool to Determine HLA Class II Epitopes from T Cell ELISpot Data.
| العنوان: | BIITE: A Tool to Determine HLA Class II Epitopes from T Cell ELISpot Data. |
|---|---|
| المؤلفون: | Lies Boelen, Patrick K O'Neill, Kathryn J Quigley, Catherine J Reynolds, Bernard Maillere, John H Robinson, Ganjana Lertmemongkolchai, Daniel M Altmann, Rosemary J Boyton, Becca Asquith |
| المصدر: | PLoS Computational Biology, Vol 12, Iss 3, p e1004796 (2016) |
| بيانات النشر: | Public Library of Science (PLoS), 2016. |
| سنة النشر: | 2016 |
| المجموعة: | LCC:Biology (General) |
| مصطلحات موضوعية: | Biology (General), QH301-705.5 |
| الوصف: | Activation of CD4+ T cells requires the recognition of peptides that are presented by HLA class II molecules and can be assessed experimentally using the ELISpot assay. However, even given an individual's HLA class II genotype, identifying which class II molecule is responsible for a positive ELISpot response to a given peptide is not trivial. The two main difficulties are the number of HLA class II molecules that can potentially be formed in a single individual (3-14) and the lack of clear peptide binding motifs for class II molecules. Here, we present a Bayesian framework to interpret ELISpot data (BIITE: Bayesian Immunogenicity Inference Tool for ELISpot); specifically BIITE identifies which HLA-II:peptide combination(s) are immunogenic based on cohort ELISpot data. We apply BIITE to two ELISpot datasets and explore the expected performance using simulations. We show this method can reach high accuracies, depending on the cohort size and the success rate of the ELISpot assay within the cohort. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1553-734X 1553-7358 |
| Relation: | https://doaj.org/toc/1553-734X; https://doaj.org/toc/1553-7358 |
| DOI: | 10.1371/journal.pcbi.1004796 |
| URL الوصول: | https://doaj.org/article/bf41c06d42fd4022a66f3f8a1f57a94c |
| رقم الأكسشن: | edsdoj.bf41c06d42fd4022a66f3f8a1f57a94c |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 1553734X 15537358 |
|---|---|
| DOI: | 10.1371/journal.pcbi.1004796 |