دورية أكاديمية
Allo-antibody production after intraarticular administration of mesenchymal stem cells (MSCs) in an equine osteoarthritis model: effect of repeated administration, MSC inflammatory stimulation, and equine leukocyte antigen (ELA) compatibility
العنوان: | Allo-antibody production after intraarticular administration of mesenchymal stem cells (MSCs) in an equine osteoarthritis model: effect of repeated administration, MSC inflammatory stimulation, and equine leukocyte antigen (ELA) compatibility |
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المؤلفون: | Laura Barrachina, Alina Cequier, Antonio Romero, Arantza Vitoria, Pilar Zaragoza, Francisco José Vázquez, Clementina Rodellar |
المصدر: | Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-12 (2020) |
بيانات النشر: | BMC, 2020. |
سنة النشر: | 2020 |
المجموعة: | LCC:Medicine (General) LCC:Biochemistry |
مصطلحات موضوعية: | Allogeneic, Immunogenicity, Joint, Horse, Humoral response, MSC priming, Medicine (General), R5-920, Biochemistry, QD415-436 |
الوصف: | Abstract Background Antibody production after allogeneic administration of mesenchymal stem cells (MSCs) could impact their clinical application. Proinflammatory priming of MSCs can potentiate their regulatory ability in vivo but increased expression of major histocompatibility complex (MHC) might augment their immunogenicity, potentially leading to immune memory thus limiting repeated allogeneic administration. This study aimed at evaluating the production of cytotoxic allo-antibodies directed against donor’s ELA (equine leukocyte antigen) in mismatched and halfmatched horses receiving repeated intraarticular administration of stimulated MSCs (MSC-primed) and unstimulated MSCs (MSC-naïve) in pathologic joints. Methods From available stored samples from a previous in vivo study, cells from one donor and serially collected sera (five time-points) from three groups of recipients were used based on their ELA haplotypes to perform microcytotoxicity assays: Group 1 recipients mismatched with the donor that received MSC-naïve (naïve-mismatched recipients); Group 2 recipients mismatched with the donor that received MSC-primed (primed-mismatched recipients); Group 3 recipients halfmatched with the donor (sharing 1/2 haplotypes) that received MSC-primed (primed-halfmatched recipients). Sera from recipients (neat, 1:2 and 1:16 dilution) were tested against target cells from the donor (cryopreserved and expanded MSC-naïve and MSC-primed) or from one animal presenting the same ELA haplotypes than the donor (fresh peripheral blood lymphocytes as control). Results One to three weeks after first MSC administration, all recipient groups produced allo-antibodies regardless of MSC received (naïve or primed) and matching degree with donor. However, secondary response after MSC re-exposure was less evident in halfmatched recipients (MSC-primed) than in mismatched ones (both MSC-naïve and MSC-primed). Recipients of MSC-primed (both mismatched and halfmatched) tended towards developing lower antibody response than MSC-naïve recipients in vivo, but MSC-primed were targeted to death in higher percentage in vitro in the microcytoxicity assay. Conclusions After first intraarticular allogeneic administration, the immunomodulatory profile of MSC-primed would have led to lower antibody production, but these antibodies would target more easily MSC-primed after second injection (re-exposure), likely because of their higher MHC expression. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1757-6512 |
Relation: | https://doaj.org/toc/1757-6512 |
DOI: | 10.1186/s13287-020-1571-8 |
URL الوصول: | https://doaj.org/article/bf82f700ba1843d5ac11558205fe3b75 |
رقم الأكسشن: | edsdoj.bf82f700ba1843d5ac11558205fe3b75 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 17576512 |
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DOI: | 10.1186/s13287-020-1571-8 |