دورية أكاديمية
Liposome-Encapsulated Berberine Alleviates Liver Injury in Type 2 Diabetes via Promoting AMPK/mTOR-Mediated Autophagy and Reducing ER Stress: Morphometric and Immunohistochemical Scoring
| العنوان: | Liposome-Encapsulated Berberine Alleviates Liver Injury in Type 2 Diabetes via Promoting AMPK/mTOR-Mediated Autophagy and Reducing ER Stress: Morphometric and Immunohistochemical Scoring |
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| المؤلفون: | Safaa I. Khater, Taghreed N. Almanaa, Doaa M. Abdel Fattah, Tarek Khamis, Mona M. Seif, Naief Dahran, Leena S. Alqahtani, Mohamed M. M. Metwally, Mahmoud Mostafa, Raghad A. Albedair, Azza I. Helal, Manal Alosaimi, Amany Abdel-Rahman Mohamed |
| المصدر: | Antioxidants, Vol 12, Iss 6, p 1220 (2023) |
| بيانات النشر: | MDPI AG, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | autophagy, AMPK/mTOR, Bclin-1, liposomal berberine, ER stress, type 2 diabetes, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | In the advanced stages of type 2 diabetes mellitus (T2DM), diabetic liver damage is a common complication that can devastate a patient’s quality of life. The present study investigated the ability of liposomal berberine (Lip-BBR) to aid in ameliorating hepatic damage and steatosis, insulin homeostasis, and regulating lipid metabolism in type 2 diabetes (T2DM) and the possible pathways by which it does so. Liver tissue microarchitectures and immunohistochemical staining were applied during the study. The rats were divided into a control non-diabetic group and four diabetic groups, which are the T2DM, T2DM-Lip-BBR (10 mg/kg b.wt), T2DM-Vildagliptin (Vild) (10 mg/kg b.wt), and T2DM-BBR-Vild (10 mg/kg b.wt + Vild (5 mg/kg b.wt) groups. The findings demonstrated that Lip-BBR treatment could restore liver tissue microarchitectures, reduce steatosis and liver function, and regulate lipid metabolism. Moreover, Lip-BBR treatment promoted autophagy via the activation of LC3-II and Bclin-1 proteins and activated the AMPK/mTOR pathway in the liver tissue of T2DM rats. Lip-BBR also activated the GLP-1 expression, which stimulated insulin biosynthesis. It decreased the endoplasmic reticulum stress by limiting the CHOP, JNK expression, oxidative stress, and inflammation. Collectively, Lip-BBR ameliorated diabetic liver injury in a T2DM rat model with its promotion activity of AMPK/mTOR-mediated autophagy and limiting ER stress. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 12061220 2076-3921 |
| Relation: | https://www.mdpi.com/2076-3921/12/6/1220; https://doaj.org/toc/2076-3921 |
| DOI: | 10.3390/antiox12061220 |
| URL الوصول: | https://doaj.org/article/bfd044e2036741fc9710743422927326 |
| رقم الأكسشن: | edsdoj.bfd044e2036741fc9710743422927326 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 12061220 20763921 |
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| DOI: | 10.3390/antiox12061220 |