دورية أكاديمية
Distinct CD16a features on human NK cells observed by flow cytometry correlate with increased ADCC
| العنوان: | Distinct CD16a features on human NK cells observed by flow cytometry correlate with increased ADCC |
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| المؤلفون: | Maria C. Rodriguez Benavente, Zainab A. Hakeem, Alexander R. Davis, Nathan B. Murray, Parastoo Azadi, Emily M. Mace, Adam W. Barb |
| المصدر: | Scientific Reports, Vol 14, Iss 1, Pp 1-14 (2024) |
| بيانات النشر: | Nature Portfolio, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | N-glycosylation, Natural killer cell, ADCC, Fc γ receptor IIIa, CD16a, Medicine, Science |
| الوصف: | Abstract Natural killer (NK) cells destroy tissue that have been opsonized with antibodies. Strategies to generate or identify cells with increased potency are expected to enhance NK cell-based immunotherapies. We previously generated NK cells with increased antibody-dependent cell mediated cytotoxicity (ADCC) following treatment with kifunensine, an inhibitor targeting mannosidases early in the N-glycan processing pathway. Kifunensine treatment also increased the antibody-binding affinity of Fc γ receptor IIIa/CD16a. Here we demonstrate that inhibiting NK cell N-glycan processing increased ADCC. We reduced N-glycan processing with the CRIPSR-CAS9 knockdown of MGAT1, another early-stage N-glycan processing enzyme, and showed that these cells likewise increased antibody binding affinity and ADCC. These experiments led to the observation that NK cells with diminished N-glycan processing capability also revealed a clear phenotype in flow cytometry experiments using the B73.1 and 3G8 antibodies binding two distinct CD16a epitopes. We evaluated this “affinity profiling” approach using primary NK cells and identified a distinct shift and differentiated populations by flow cytometry that correlated with increased ADCC. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-024-58541-6 |
| URL الوصول: | https://doaj.org/article/af037a6d23824e0396d24c7b526b7a61 |
| رقم الأكسشن: | edsdoj.f037a6d23824e0396d24c7b526b7a61 |
| قاعدة البيانات: | Directory of Open Access Journals |
Find this article in full text from Springer Verlag. 
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-024-58541-6 |