دورية أكاديمية
Development of canine PD-1/PD-L1 specific monoclonal antibodies and amplification of canine T cell function.
| العنوان: | Development of canine PD-1/PD-L1 specific monoclonal antibodies and amplification of canine T cell function. |
|---|---|
| المؤلفون: | Jin Wook Choi, Sita S Withers, Hong Chang, Justin A Spanier, Victoria L De La Trinidad, Harmanpreet Panesar, Brian T Fife, Roger Sciammas, Ellen E Sparger, Peter F Moore, Michael S Kent, Robert B Rebhun, Stephen J McSorley |
| المصدر: | PLoS ONE, Vol 15, Iss 7, p e0235518 (2020) |
| بيانات النشر: | Public Library of Science (PLoS), 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | Interruption of the programmed death 1 (PD-1) / programmed death ligand 1 (PD-L1) pathway is an established and effective therapeutic strategy in human oncology and holds promise for veterinary oncology. We report the generation and characterization of monoclonal antibodies specific for canine PD-1 and PD-L1. Antibodies were initially assessed for their capacity to block the binding of recombinant canine PD-1 to recombinant canine PD-L1 and then ranked based on efficiency of binding as judged by flow cytometry. Selected antibodies were capable of detecting PD-1 and PD-L1 on canine tissues by flow cytometry and Western blot. Anti-PD-L1 worked for immunocytochemistry and anti-PD-1 worked for immunohistochemistry on formalin-fixed paraffin embedded canine tissues, suggesting the usage of this antibody with archived tissues. Additionally, anti-PD-L1 (JC071) revealed significantly increased PD-L1 expression on canine monocytes after stimulation with peptidoglycan or lipopolysaccharide. Together, these antibodies display specificity for the natural canine ligand using a variety of potential diagnostic applications. Importantly, multiple PD-L1-specific antibodies amplified IFN-γ production in a canine peripheral blood mononuclear cells (PBMC) concanavlin A (Con A) stimulation assay, demonstrating functional activity. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0235518 |
| URL الوصول: | https://doaj.org/article/f05654296edd40d39bd096f9fc678bd8 |
| رقم الأكسشن: | edsdoj.f05654296edd40d39bd096f9fc678bd8 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
|---|---|
| DOI: | 10.1371/journal.pone.0235518 |