Acidic walnut green husk polysaccharide with weight average molecular weight of 5.101 kDa was isolated and designated WGHP-2, which was composed of galacturonic acid (69.47 %), galactose (11.18 %), rhamnose (8.67 %), arabinose (3.96 %), glucose (2.21 %), glucuronic acid (2.28 %), xylose (0.83 %), fucose (0.81 %) and mannose (0.59 %). The main glycosidic linkage types of WGHP-2 were → 4-α-D-Glcp-(1→, →4-α-D-GalpA-(1→, t-β-D-GlcpA-(1→, →2-β-D-Rhap-(1→, t-α-D-Galp-(1→, →4-α-D-Galp-(1 → and → 2,4)-α-D-Manp(1 →. Immunostimulating assays showed that WGHP-2 could significantly promote the proliferation of RAW264.7 cells, strengthen the phagocytic activity, and up-regulate the production and release of NO, ROS, TNF-α, INF-β and IL-6. Western-blot analysis indicated that MAPK and PI3K/Akt pathways were involved in the activation of macrophages induced by WGHP-2. Overall, our study suggests that WGHP-2 has the potential to be developed as a functional food with good immunostimulatory activity.
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