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Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells

التفاصيل البيبلوغرافية
العنوان: Effect of Non-Thermal Atmospheric Pressure Plasma on Differentiation Potential of Human Deciduous Dental Pulp Fibroblast-like Cells
المؤلفون: Masae Okuno, Sho Aoki, Saki Kawai, Rie Imataki, Yoko Abe, Kyoko Harada, Kenji Arita
المصدر: Applied Sciences, Vol 11, Iss 21, p 10119 (2021)
بيانات النشر: MDPI AG, 2021.
سنة النشر: 2021
المجموعة: LCC:Technology
LCC:Engineering (General). Civil engineering (General)
LCC:Biology (General)
LCC:Physics
LCC:Chemistry
مصطلحات موضوعية: non-thermal atmospheric pressure plasma (NTAPP), human deciduous dental pulp fibroblast-like cells, regenerative medicine, Technology, Engineering (General). Civil engineering (General), TA1-2040, Biology (General), QH301-705.5, Physics, QC1-999, Chemistry, QD1-999
الوصف: Human mesenchymal stem cells can differentiate into various cell types and are useful for applications in regenerative medicine. Previous studies indicated that dental pulp exfoliated from deciduous teeth is a valuable alternative for dental tissue engineering because it contains stem cells with a relatively high proliferation rate. For clinical application, it is necessary to rapidly obtain a sufficient number of cells in vitro and maintain their undifferentiated state; however, the abundance of stem cells in the dental pulp tissue is limited. Non-thermal atmospheric pressure plasma (NTAPP) has been applied in regenerative medicine because it activates cell proliferation. Here, we examined the effects of NTAPP to activate the proliferation of human deciduous dental pulp fibroblast-like cells (hDDPFs) in vitro. Compared with untreated cells, NTAPP increased cell proliferation by 1.3-fold, significantly upregulated well-known pluripotent genes for stemness (e.g., Oct4, Sox2, and Nanog), and activated the expression of stem cell-specific surface markers (e.g., CD105). Overall, NTAPP activated the proliferation of various mesodermal-derived human adult stem cells while maintaining their pluripotency and stemness. In conclusion, NTAPP is a potential tool to expand the population of various adult stem cells in vitro for medical applications.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2076-3417
Relation: https://www.mdpi.com/2076-3417/11/21/10119; https://doaj.org/toc/2076-3417
DOI: 10.3390/app112110119
URL الوصول: https://doaj.org/article/f082595ba1d6426d90fdcaaa1d370f7b
رقم الأكسشن: edsdoj.f082595ba1d6426d90fdcaaa1d370f7b
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20763417
DOI:10.3390/app112110119