دورية أكاديمية
Expressions of matrix metalloproteinases 1 and 3 and their tissue inhibitors in the conjunctival tissue and fibroblasts cultured from conjunctivochalasis
العنوان: | Expressions of matrix metalloproteinases 1 and 3 and their tissue inhibitors in the conjunctival tissue and fibroblasts cultured from conjunctivochalasis |
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المؤلفون: | Min-Hong Xiang, Xing-Ru Zhang, Zhen-Yong Zhang, Qing-Song Li, Han-Min Wang, Zhu-Mei Han, Huan-Ming Zhou, Yuan-Ling Jia, Xing-Xing Chen |
المصدر: | International Journal of Ophthalmology, Vol 10, Iss 4, Pp 555-559 (2017) |
بيانات النشر: | Press of International Journal of Ophthalmology (IJO PRESS), 2017. |
سنة النشر: | 2017 |
المجموعة: | LCC:Ophthalmology |
مصطلحات موضوعية: | 559, conjunctivochalasis, relaxed conjunctiva, fibroblast, matrix metalloproteinase, tissue inhibitor of matrix metalloproteinase, Ophthalmology, RE1-994 |
الوصف: | AIM: To investigate the expression of matrix metalloproteinases 1 and 3 (MMP-1 and MMP-3) and their tissue inhibitors of metalloproteinases 1 and 3 (TIMP-1 and TIMP-3) in the conjunctiva of eyes with conjunctivochalasis (CCh). METHODS: The conjunctival tissue was obtained from the CCh patients and controls, the MMPs/TIMPs expression concentration was determined by enzyme-linked immuno-sorbent assay (ELISA) and immunofluorescence staining. The expression levels of MMPs/TIMPs in the CCh fibro-blasts were determined by analyzing its concentration in the cellular supernatant that was abstracted from the in vitro cultured CCh fibroblasts. RESULTS: MMP-1 and MMP-3 levels determined by ELISA were both significantly higher in the CCh group than that in the control group (P=0.042, 0.022, respectively), so was the levels of TIMP-1 (P=0.010). No significant difference in the expression of TIMP-3 in conjunctiva was found between the two groups (P=0.298). The expression of MMP-1 and MMP-3 were both up-regulated significantly in the CCh group (P=0.040, 0.001, respectively) on immuno-fluorescence staining. MMP-1 and MMP-3 expression in the fibroblasts were both significantly higher in the CCh group than that in the control group (P=0.027, 0.001, respectively), while neither the TIMP-1 nor TIMP-3 expression was significantly different between the two groups (P=0.421, 0.237, respectively). CONCLUSION: The overexpression of MMP-1 and MMP-3 in conjunctival tissue and fibroblasts may play an important role in the pathogenesis and development of CCh. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 2222-3959 2227-4898 |
Relation: | http://www.ijo.cn/en_publish/2017/4/20170409.pdf; https://doaj.org/toc/2222-3959; https://doaj.org/toc/2227-4898 |
DOI: | 10.18240/ijo.2017.04.09 |
URL الوصول: | https://doaj.org/article/f09bffc9cf5140429c6a3397544ae41c |
رقم الأكسشن: | edsdoj.f09bffc9cf5140429c6a3397544ae41c |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 22223959 22274898 |
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DOI: | 10.18240/ijo.2017.04.09 |