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Hypoxia-adaptation involves mitochondrial metabolic depression and decreased ROS leakage.

التفاصيل البيبلوغرافية
العنوان: Hypoxia-adaptation involves mitochondrial metabolic depression and decreased ROS leakage.
المؤلفون: Sameh S Ali, Mary Hsiao, Huiwen W Zhao, Laura L Dugan, Gabriel G Haddad, Dan Zhou
المصدر: PLoS ONE, Vol 7, Iss 5, p e36801 (2012)
بيانات النشر: Public Library of Science (PLoS), 2012.
سنة النشر: 2012
المجموعة: LCC:Medicine
LCC:Science
مصطلحات موضوعية: Medicine, Science
الوصف: Through long-term laboratory selection, we have generated a Drosophila melanogaster population that tolerates severe, normally lethal, level of hypoxia. This strain lives perpetually under severe hypoxic conditions (4% O(2)). In order to shed light on the mechanisms involved in this adaptation, we studied the respiratory function of isolated mitochondria from the thorax of hypoxia-adapted flies (AF) using polarographic oxygen consumption while monitoring superoxide generation by electron paramagnetic resonance (EPR) techniques. AF mitochondria exhibited a significant 30% decrease in respiratory rate during state 3, while enhancing the resting respiratory rate during State 4-oligo by 220%. The activity of individual electron transport complexes I, II and III were 107%, 65%, and 120% in AF mitochondria as compared to those isolated from control flies. The sharp decrease in complex II activity and modest increase in complexes I and III resulted in >60% reduction in superoxide leakage from AF mitochondria during both NAD(+)-linked state 3 and State 4-oligo respirations. These results provide evidence that flies with mitochondria exhibiting decreased succinate dehydrogenase activity and reduced superoxide leakage give flies an advantage for survival in long-term hypoxia.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1932-6203
Relation: http://europepmc.org/articles/PMC3344937?pdf=render; https://doaj.org/toc/1932-6203
DOI: 10.1371/journal.pone.0036801
URL الوصول: https://doaj.org/article/f0c459cf6bcd4c2185ab7aa28ade7757
رقم الأكسشن: edsdoj.f0c459cf6bcd4c2185ab7aa28ade7757
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19326203
DOI:10.1371/journal.pone.0036801