دورية أكاديمية
Intra- and inter-protein couplings of backbone motions underlie protein thiol-disulfide exchange cascade
| العنوان: | Intra- and inter-protein couplings of backbone motions underlie protein thiol-disulfide exchange cascade |
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| المؤلفون: | Wenbo Zhang, Xiaogang Niu, Jienv Ding, Yunfei Hu, Changwen Jin |
| المصدر: | Scientific Reports, Vol 8, Iss 1, Pp 1-14 (2018) |
| بيانات النشر: | Nature Portfolio, 2018. |
| سنة النشر: | 2018 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Thiol-disulfide Exchange Reactions, Chemical Exchange Saturation Transfer (CEST), Arscia, CEST Experiments, CEST Data, Medicine, Science |
| الوصف: | Abstract The thioredoxin (Trx)-coupled arsenate reductase (ArsC) is a family of enzymes that catalyzes the reduction of arsenate to arsenite in the arsenic detoxification pathway. The catalytic cycle involves a series of relayed intramolecular and intermolecular thiol-disulfide exchange reactions. Structures at different reaction stages have been determined, suggesting significant conformational fluctuations along the reaction pathway. Herein, we use two state-of-the-art NMR methods, the chemical exchange saturation transfer (CEST) and the CPMG-based relaxation dispersion (CPMG RD) experiments, to probe the conformational dynamics of B. subtilis ArsC in all reaction stages, namely the enzymatic active reduced state, the intra-molecular C10–C82 disulfide-bonded intermediate state, the inactive oxidized state, and the inter-molecular disulfide-bonded protein complex with Trx. Our results reveal highly rugged energy landscapes in the active reduced state, and suggest global collective motions in both the C10–C82 disulfide-bonded intermediate and the mixed-disulfide Trx-ArsC complex. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2045-2322 |
| Relation: | https://doaj.org/toc/2045-2322 |
| DOI: | 10.1038/s41598-018-33766-4 |
| URL الوصول: | https://doaj.org/article/f107f36c806848fe9ba43bb176dbaf44 |
| رقم الأكسشن: | edsdoj.f107f36c806848fe9ba43bb176dbaf44 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 20452322 |
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| DOI: | 10.1038/s41598-018-33766-4 |