دورية أكاديمية
Inactivation of RIP3 kinase sensitizes to 15LOX/PEBP1-mediated ferroptotic death
| العنوان: | Inactivation of RIP3 kinase sensitizes to 15LOX/PEBP1-mediated ferroptotic death |
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| المؤلفون: | Andrew M. Lamade, Limin Wu, Haider H. Dar, Heather L. Mentrup, Indira H. Shrivastava, Michael W. Epperly, Claudette M. St Croix, Yulia Y. Tyurina, Tamil S. Anthonymuthu, Qin Yang, Aleksandr A. Kapralov, Zhentai Huang, Gaowei Mao, Andrew A. Amoscato, Zachary E. Hier, Margarita A. Artyukhova, Galina Shurin, Joel C. Rosenbaum, Peter J. Gough, John Bertin, Andrew P. VanDemark, Simon C. Watkins, Kevin P. Mollen, Ivet Bahar, Joel S. Greenberger, Valerian E. Kagan, Michael J. Whalen, Hülya Bayır |
| المصدر: | Redox Biology, Vol 50, Iss , Pp 102232- (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | Lipid signaling, Whole body irradiation, Traumatic brain injury, Regulated necrosis, Necroptotic death, Ferroptotic death, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | Ferroptosis and necroptosis are two pro-inflammatory cell death programs contributing to major pathologies and their inhibition has gained attention to treat a wide range of disease states. Necroptosis relies on activation of RIP1 and RIP3 kinases. Ferroptosis is triggered by oxidation of polyunsaturated phosphatidylethanolamines (PUFA-PE) by complexes of 15-Lipoxygenase (15LOX) with phosphatidylethanolamine-binding protein 1 (PEBP1). The latter, also known as RAF kinase inhibitory protein, displays promiscuity towards multiple proteins. In this study we show that RIP3 K51A kinase inactive mice have increased ferroptotic burden and worse outcome after irradiation and brain trauma rescued by anti-ferroptotic compounds Liproxstatin-1 and Ferrostatin 16-86. Given structural homology between RAF and RIP3, we hypothesized that PEBP1 acts as a necroptosis-to-ferroptosis switch interacting with either RIP3 or 15LOX. Using genetic, biochemical, redox lipidomics and computational approaches, we uncovered that PEBP1 complexes with RIP3 and inhibits necroptosis. Elevated expression combined with higher affinity enables 15LOX to pilfer PEBP1 from RIP3, thereby promoting PUFA-PE oxidation and ferroptosis which sensitizes Rip3K51A/K51A kinase-deficient mice to total body irradiation and brain trauma. This newly unearthed PEBP1/15LOX-driven mechanism, along with previously established switch between necroptosis and apoptosis, can serve multiple and diverse cell death regulatory functions across various human disease states. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2213-2317 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2213231722000040; https://doaj.org/toc/2213-2317 |
| DOI: | 10.1016/j.redox.2022.102232 |
| URL الوصول: | https://doaj.org/article/f183853d46e94325b02ce4c0422c4965 |
| رقم الأكسشن: | edsdoj.f183853d46e94325b02ce4c0422c4965 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 22132317 |
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| DOI: | 10.1016/j.redox.2022.102232 |