دورية أكاديمية
Feminization of channel catfish with 17β-oestradiol involves methylation and expression of a specific set of genes independent of the sex determination region
| العنوان: | Feminization of channel catfish with 17β-oestradiol involves methylation and expression of a specific set of genes independent of the sex determination region |
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| المؤلفون: | Wenwen Wang, Suxu Tan, Yujia Yang, Tao Zhou, De Xing, Baofeng Su, Jinhai Wang, Shangjia Li, Mei Shang, Dongya Gao, Rex Dunham, Zhanjiang Liu |
| المصدر: | Epigenetics, Vol 17, Iss 12, Pp 1820-1837 (2022) |
| بيانات النشر: | Taylor & Francis Group, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Genetics |
| مصطلحات موضوعية: | sex reversal, methylation, oestrogen, transcription, sex differentiation, teleost, Genetics, QH426-470 |
| الوصف: | Exogenous oestrogen 17β-oestradiol (E2) has been shown to effectively induce feminization in teleosts. However, the molecular mechanisms underlying the process remain unclear. Here, we determined global DNA methylation and gene expression profiles of channel catfish (Ictalurus punctatus) during early sex differentiation after E2 treatment. Overall, the levels of global DNA methylation after E2 treatment were not significantly different from those of controls. However, a specific set of genes were differentially methylated, which included many sex differentiation-related pathways, such as MARK signalling, adrenergic signalling, Wnt signalling, GnRH signalling, ErbB signalling, and ECM–receptor interactions. Many genes involved in these pathways were also differentially expressed after E2 treatment. Specifically, E2 treatments resulted in upregulation of female-related genes and downregulation of male-related genes in genetic males during sex reversal. However, E2-induced sex reversal did not cause sex-specific changes in methylation profiles or gene expression within the sex determination region (SDR) on chromosome 4, suggesting that E2-induced sex reversal was a downstream process independent of the sex determination process that was regulated by sex-specific methylation within the SDR. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1559-2294 1559-2308 15592294 |
| Relation: | https://doaj.org/toc/1559-2294; https://doaj.org/toc/1559-2308 |
| DOI: | 10.1080/15592294.2022.2086725 |
| URL الوصول: | https://doaj.org/article/f26409086f974a40a00773aa26fd563d |
| رقم الأكسشن: | edsdoj.f26409086f974a40a00773aa26fd563d |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 15592294 15592308 |
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| DOI: | 10.1080/15592294.2022.2086725 |