دورية أكاديمية
Minimal residual disease detection in Tunisian B-acute lymphoblastic leukemia based on immunoglobulin gene rearrangements
| العنوان: | Minimal residual disease detection in Tunisian B-acute lymphoblastic leukemia based on immunoglobulin gene rearrangements |
|---|---|
| المؤلفون: | S. Besbes, W.S. Hamadou, M.L. Boulland, Y.B. Youssef, B. Achour, H. Regaieg, A. Khelif, T. Fest, Z. Soua |
| المصدر: | Brazilian Journal of Medical and Biological Research, Vol 50, Iss 1 |
| بيانات النشر: | Associação Brasileira de Divulgação Científica. |
| المجموعة: | LCC:Medicine (General) LCC:Biology (General) |
| مصطلحات موضوعية: | IGH gene, IGK gene, B-ALL minimal residual disease (MRD), RQ-PCR, Medicine (General), R5-920, Biology (General), QH301-705.5 |
| الوصف: | IGH gene rearrangement and IGK-Kde gene deletion can be used as molecular markers for the assessment of B lineage acute lymphoblastic leukemia (B-ALL). Minimal residual disease detected based on those markers is currently the most reliable prognosis factor in B-ALL. The aim of this study was to use clonal IGH/IGK-Kde gene rearrangements to confirm B-ALL diagnosis and to evaluate the treatment outcome of Tunisian leukemic patients by monitoring the minimal residual disease (MRD) after induction chemotherapy. Seventeen consecutive newly diagnosed B-ALL patients were investigated by multiplex PCR assay and real time quantitative PCR according to BIOMED 2 conditions. The vast majority of clonal VH-JH rearrangements included VH3 gene. For IGK deletion, clonal VK1f/6-Kde recombinations were mainly identified. These rearrangements were quantified to follow-up seven B-ALL after induction using patient-specific ASO. Four patients had an undetectable level of MRD with a sensitivity of up to 10-5. This molecular approach allowed identification of prognosis risk group and adequate therapeutic decision. The IGK-Kde and IGH gene rearrangements might be used for diagnosis and MRD monitoring of B-ALL, introduced for the first time in Tunisian laboratories. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1414-431X 1414-431x |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000100703&lng=en&tlng=en; https://doaj.org/toc/1414-431X |
| DOI: | 10.1590/1414-431x20165426 |
| URL الوصول: | https://doaj.org/article/f3024653e74e4321b6a6130fa0d9c8c0 |
| رقم الأكسشن: | edsdoj.f3024653e74e4321b6a6130fa0d9c8c0 |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 1414431X 1414431x |
|---|---|
| DOI: | 10.1590/1414-431x20165426 |