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SMRT sequencing revealed the diversity and characteristics of defective interfering RNAs in influenza A (H7N9) virus infection

التفاصيل البيبلوغرافية
العنوان: SMRT sequencing revealed the diversity and characteristics of defective interfering RNAs in influenza A (H7N9) virus infection
المؤلفون: Wing-Yu Lui, Chun-Kit Yuen, Can Li, Wan Man Wong, Pak-Yin Lui, Chi-Ho Lin, Kwok-Hung Chan, Hanjun Zhao, Honglin Chen, Kelvin K. W. To, Anna J. X Zhang, Kwok-Yung Yuen, Kin-Hang Kok
المصدر: Emerging Microbes and Infections, Vol 8, Iss 1, Pp 662-674 (2019)
بيانات النشر: Taylor & Francis Group, 2019.
سنة النشر: 2019
المجموعة: LCC:Infectious and parasitic diseases
LCC:Microbiology
مصطلحات موضوعية: Avian influenza A/H7N9 virus, defective interfering viral genome, Single Molecule Real Time sequencing, Illumina sequencing, Infectious and parasitic diseases, RC109-216, Microbiology, QR1-502
الوصف: ABSTRACTInfluenza defective interfering (DI) particles are replication-incompetent viruses carrying large internal deletion in the genome. The loss of essential genetic information causes abortive viral replication, which can be rescued by co-infection with a helper virus that possesses an intact genome. Despite reports of DI particles present in seasonal influenza A H1N1 infections, their existence in human infections by the avian influenza A viruses, such as H7N9, has not been studied. Here we report the ubiquitous presence of DI-RNAs in nasopharyngeal aspirates of H7N9-infected patients. Single Molecule Real Time (SMRT) sequencing was first applied and long-read sequencing analysis showed that a variety of H7N9 DI-RNA species were present in the patient samples and human bronchial epithelial cells. In several abundantly expressed DI-RNA species, long overlapping sequences have been identified around at the breakpoint region and the other side of deleted region. Influenza DI-RNA is known as a defective viral RNA with single large internal deletion. Beneficial to the long-read property of SMRT sequencing, double and triple internal deletions were identified in half of the DI-RNA species. In addition, we examined the expression of DI-RNAs in mice infected with sublethal dose of H7N9 virus at different time points. Interestingly, DI-RNAs were abundantly expressed as early as day 2 post-infection. Taken together, we reveal the diversity and characteristics of DI-RNAs found in H7N9-infected patients, cells and animals. Further investigations on this overwhelming generation of DI-RNA may provide important insights into the understanding of H7N9 viral replication and pathogenesis.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 22221751
2222-1751
Relation: https://doaj.org/toc/2222-1751
DOI: 10.1080/22221751.2019.1611346
URL الوصول: https://doaj.org/article/f33b858fe2994e339e8ecd9c7eb0e18f
رقم الأكسشن: edsdoj.f33b858fe2994e339e8ecd9c7eb0e18f
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:22221751
DOI:10.1080/22221751.2019.1611346