دورية أكاديمية
Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran
العنوان: | Molecular and Morphological Data Confirmed First Record of Abbreviata kazakhstanica Markov and Paraskiv, 1956 (Spirurida: Physalopteridea) in Iran |
---|---|
المؤلفون: | Morteza Ghanbari Johkool, Aida Vafae Eslahi, Milad Badri, Elham Hooshmand, Majid Pirestani, Daniel Jablonski, Rasool Jafari, Abdolhossein Dalimi |
المصدر: | Iranian Journal of Parasitology, Vol 16, Iss 4, Pp 686-691 (2021) |
بيانات النشر: | Tehran University of Medical Sciences, 2021. |
سنة النشر: | 2021 |
المجموعة: | LCC:Infectious and parasitic diseases |
مصطلحات موضوعية: | abbreviata kazakhstanica, molecular identification, pseudopus apodus, physalopteridea, iran, Infectious and parasitic diseases, RC109-216 |
الوصف: | Background: The genus Abbreviata (Spirurida: Physalopteridea) currently contains 47 species. Physalopteridae nematodes infect a large number of vertebrates, including mammals, birds, reptiles and amphibians. The current study is a report of the first morphological and molecular identification of A. kazakhstanica (Spirurida: Physalopteridea) in Pseudopus apodus in Iran. Methods: Eleven road-killed P. apodus, were collected from, Iran during 2016-2018. The nematodes were isolated from stomach. After morphological study, the genomic DNA of the parasites was extracted using CTAB method. The DNA was used for PCR amplification of cytochrome c oxidase subunit I (cox1). The PCR products were sequenced, the sequence data were analyzed and multiple alignments were conducted using the Clustal Omega. Results: After detailed microscopic examination, the A. kazakhstanica was identified. The cox1 sequences confirmed the species of helminth. The new sequences of A. kazakhstanica were submitted to GenBank under the accession number MK578751-2. Conclusion: Regarding the limited data on parasitological status of Iranian reptiles, more specific and comprehensive investigations are needed to identify the parasitic fauna. |
نوع الوثيقة: | article |
وصف الملف: | electronic resource |
اللغة: | English |
تدمد: | 1735-7020 2008-238X |
Relation: | https://ijpa.tums.ac.ir/index.php/ijpa/article/view/2895; https://doaj.org/toc/1735-7020; https://doaj.org/toc/2008-238X |
URL الوصول: | https://doaj.org/article/f38760580aa247878461e6c39b071057 |
رقم الأكسشن: | edsdoj.f38760580aa247878461e6c39b071057 |
قاعدة البيانات: | Directory of Open Access Journals |
تدمد: | 17357020 2008238X |
---|