Institute of Basic Medical Sciences and Peking Union Medical College Hospital, Chinese Academy of Medical Sciences / Peking Union Medical College., 2020.
Objective To characterize the biological features of CD146+ cells from umbilical cord mesenchymal stem cells(UCMSCs), adipose-derived mesenchymal stem cells(AMSCs) and bone marrow mesenchymal stem cells(BM-MSCs). Methods MSCs from three tissues were sorted by magnetically activated cell sorting(MACS) to obtain highly purified CD146+ subpopulations. The phenotypes and ultra-structures of these three MSC were analyzed by flow-cytometry and transmission electron microscopy. The differentiation abilities were tested by adipogenic and osteogenic inductions. The expression of genes relative to adipogenesis, osteogenesis, stemness or angiogenesis were determined by RT-qPCR. Results CD146+ cells derived from three kinds of tissues had similar ultra-structures and the ability of angiogenesis. CD146+ AMSCs expressed highest levels of the OCT-4, SOX2 and NANOG genes.Under the same induction conditions, CD146+ AMSCs showed higher potential for adipogenesis and CD146+BM-MSCs showed higher potential for osteogenesis.However, CD146+ UMSCs had little ability for adipogenesis and osteogenesis. All of CD146+ MSCs from 3 tissues expressed BFGF, VEGF, Ang-1 and EGF although CD146+ BM-MSCs had stronger ability of angiogenesis in vitro. Conclusions CD146+ MSCs derived from the 3 sources exhibit different biological characteristics.These results provide a useful biological basis for further studies relative to application of MSCs.
