دورية أكاديمية
Bioinspired porous microspheres for sustained hypoxic exosomes release and vascularized bone regeneration
| العنوان: | Bioinspired porous microspheres for sustained hypoxic exosomes release and vascularized bone regeneration |
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| المؤلفون: | Yike Gao, Zuoying Yuan, Xiaojing Yuan, Zhuo Wan, Yingjie Yu, Qi Zhan, Yuming Zhao, Jianmin Han, Jianyong Huang, Chunyang Xiong, Qing Cai |
| المصدر: | Bioactive Materials, Vol 14, Iss , Pp 377-388 (2022) |
| بيانات النشر: | KeAi Communications Co., Ltd., 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Materials of engineering and construction. Mechanics of materials LCC:Biology (General) |
| مصطلحات موضوعية: | Hypoxic exosomes, Stem cells from human exfoliated deciduous teeth, Porous microsphere, Sustained release, Vascularized bone regeneration, Materials of engineering and construction. Mechanics of materials, TA401-492, Biology (General), QH301-705.5 |
| الوصف: | Exosomes derived from mesenchymal stem cells (MSCs) have demonstrated regenerative potential for cell-free bone tissue engineering, nevertheless, certain challenges, including the confined therapeutic potency of exosomes and ineffective delivery method, are still persisted. Here, we confirmed that hypoxic precondition could induce enhanced secretion of exosomes from stem cells from human exfoliated deciduous teeth (SHEDs) via comprehensive proteomics analysis, and the corresponding hypoxic exosomes (H-Exo) exhibited superior potential in promoting cellular angiogenesis and osteogenesis via the significant up-regulation in focal adhesion, VEGF signaling pathway, and thyroid hormone synthesis. Then, we developed a platform technology enabling the effective delivery of hypoxic exosomes with sustained release kinetics to irregular-shaped bone defects via injection. This platform is based on a simple adsorbing technique, where exosomes are adsorbed onto the surface of injectable porous poly(lactide-co-glycolide) (PLGA) microspheres with bioinspired polydopamine (PDA) coating (PMS-PDA microspheres). The PMS-PDA microspheres could effectively adsorb exosomes, show sustained release of H-Exo for 21 days with high bioactivity, and induce vascularized bone regeneration in 5-mm rat calvarial defect. These findings indicate that the hypoxic precondition and PMS-PDA porous microsphere-based exosome delivery are efficient in inducing tissue regeneration, hence facilitating the clinical translation of exosome-based therapy. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2452-199X 19117914 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2452199X22000561; https://doaj.org/toc/2452-199X |
| DOI: | 10.1016/j.bioactmat.2022.01.041 |
| URL الوصول: | https://doaj.org/article/f4b81ea56e2e4789a19117914e7d7671 |
| رقم الأكسشن: | edsdoj.f4b81ea56e2e4789a19117914e7d7671 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 2452199X 19117914 |
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| DOI: | 10.1016/j.bioactmat.2022.01.041 |