دورية أكاديمية
Electrochemical potential of the inner mitochondrial membrane and Ca(2+) homeostasis of myometrium cells
| العنوان: | Electrochemical potential of the inner mitochondrial membrane and Ca(2+) homeostasis of myometrium cells |
|---|---|
| المؤلفون: | Yu. V. Danylovych, S. A. Karakhim, H. V. Danylovych, O. V. Kolomiets, S. O. Kosterin |
| المصدر: | The Ukrainian Biochemical Journal, Vol 87, Iss 5, Pp 61-71 (2015) |
| بيانات النشر: | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Biochemistry LCC:Medicine LCC:Biology (General) |
| مصطلحات موضوعية: | calcium, calcium homeostasis, electrochemical potential of mitochondrial membrane, mitochondria, nitric oxide, Biochemistry, QD415-436, Medicine, Biology (General), QH301-705.5 |
| الوصف: | We demonstrated using Ca2+-sensitive fluorescent probe, mitochondria binding dyes, and confocal laser scanning microscopy, that elimination of electrochemical potential of uterus myocytes’ inner mitochondrial membrane by a protonophore carbonyl cyanide m-chlorophenyl hуdrazone (10 μM), and by a respiratory chain complex IV inhibitor sodium azide (1 mM) is associated with substantial increase of Ca2+ concentration in myoplasm in the case of the protonophore effect only, but not in the case of the azide effect. In particular, with the use of nonyl acridine orange, a mitochondria-specific dye, and 9-aminoacridine, an agent that binds to membrane compartments in the presence of proton gradient, we showed that both the protonophore and the respiratory chain inhibitor cause the proton gradient on mitochondrial inner membrane to dissipate when introduced into incubation medium. We also proved with the help of 3,3′-dihexyloxacarbocyanine, a potential-sensitive carbocyanine-derived fluorescent probe, that the application of these substances results in dissipation of the membrane’s electrical potential. The elimination of mitochondrial electrochemical potential by carbonyl cyanide m-chlorophenyl hуdrazone causes substantial increase in fluorescence of Ca2+-sensitive Fluo-4 AM dye in myoplasm of smooth muscle cells. The results obtained were qualitatively confirmed with flow cytometry of mitochondria isolated through differential centrifugation and loaded with Fluo-4 AM. Particularly, Ca2+ matrix influx induced by addition of the exogenous cation is totally inhibited by carbonyl cyanide m-chlorophenyl hydrazone. Therefore, using two independent fluorometric methods, namely confocal laser scanning microscopy and flow cytometry, with Ca2+-sensitive Fluo-4 AM fluorescent probe, we proved on the models of freshly isolated myocytes and uterus smooth muscle mitochondria isolated by differential centrifugation sedimentation that the electrochemical gradient of inner membrane is an important component of mechanisms that regulate Ca2+ homeostasis in myometrium cells. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2409-4943 2413-5003 |
| Relation: | http://ukrbiochemjournal.org/wp-content/uploads/2015/10/Danylovych_5_15.pdf; https://doaj.org/toc/2409-4943; https://doaj.org/toc/2413-5003 |
| DOI: | 10.15407/ubj87.05.061 |
| URL الوصول: | https://doaj.org/article/f506cec2a12e429db58440cdf7c486bc |
| رقم الأكسشن: | edsdoj.f506cec2a12e429db58440cdf7c486bc |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24094943 24135003 |
|---|---|
| DOI: | 10.15407/ubj87.05.061 |