دورية أكاديمية
Exploration study of indigenous civet (Paradoxorus hermaphroditus) bacteria: isolates characterization and molecular identification
| العنوان: | Exploration study of indigenous civet (Paradoxorus hermaphroditus) bacteria: isolates characterization and molecular identification |
|---|---|
| المؤلفون: | Murna Muzaifa, Yusya Abubakar, Febriani Febriani, Amhar Abubakar |
| المصدر: | Agrointek, Vol 17, Iss 1, Pp 78-85 (2023) |
| بيانات النشر: | Universitas Trunojoyo Madura, 2023. |
| سنة النشر: | 2023 |
| المجموعة: | LCC:Agriculture |
| مصطلحات موضوعية: | bacteria, civet coffee, molecular identification, 16 rrna, Agriculture |
| الوصف: | Biochemistry of civet coffee fermentation in the digestive tract of civets until now is not fully known. This study aims to isolation, characterization and identification of bacteria molecularly from the civet's digestive tract. Analysis of bacteria begins with the civet surgery to obtain fluid from three parts of the civet's digestive tract. Isolation of bacteria conducted by pour plate method using nutrient agar media. Single and different colonies (shape, colour, margin, elevation, size) were separated and purified by streaking method. The pure isolate was further characterized biochemically. The biochemical characteristics of the bacteria observed were Gram staining, catalase, protease and pectinase activity. Identification of the bacteria was carried out molecularly by identifying the 16S rRNA gene. The results of molecular identification showed that the bacteria from the civet were identified as Alcaligenes faecalis strain LHW 1749, Ochrobactrum anthropi, Stenotrophomonas sp. 262 and Bacillus sp. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English Indonesian |
| تدمد: | 1907-8056 2527-5410 |
| Relation: | https://journal.trunojoyo.ac.id/agrointek/article/view/13841; https://doaj.org/toc/1907-8056; https://doaj.org/toc/2527-5410 |
| DOI: | 10.21107/agrointek.v17i1.13841 |
| URL الوصول: | https://doaj.org/article/df56eb65bc5a4e8da26775d3408302fb |
| رقم الأكسشن: | edsdoj.f56eb65bc5a4e8da26775d3408302fb |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19078056 25275410 |
|---|---|
| DOI: | 10.21107/agrointek.v17i1.13841 |