دورية أكاديمية
Doxycycline alters metabolism and proliferation of human cell lines.
| العنوان: | Doxycycline alters metabolism and proliferation of human cell lines. |
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| المؤلفون: | Ethan Ahler, William J Sullivan, Ashley Cass, Daniel Braas, Autumn G York, Steven J Bensinger, Thomas G Graeber, Heather R Christofk |
| المصدر: | PLoS ONE, Vol 8, Iss 5, p e64561 (2013) |
| بيانات النشر: | Public Library of Science (PLoS), 2013. |
| سنة النشر: | 2013 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | The tetracycline antibiotics are widely used in biomedical research as mediators of inducible gene expression systems. Despite many known effects of tetracyclines on mammalian cells-including inhibition of the mitochondrial ribosome-there have been few reports on potential off-target effects at concentrations commonly used in inducible systems. Here, we report that in human cell lines, commonly used concentrations of doxycycline change gene expression patterns and concomitantly shift metabolism towards a more glycolytic phenotype, evidenced by increased lactate secretion and reduced oxygen consumption. We also show that these concentrations are sufficient to slow proliferation. These findings suggest that researchers using doxycycline in inducible expression systems should design appropriate controls to account for potential confounding effects of the drug on cellular metabolism. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC3669316?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0064561 |
| URL الوصول: | https://doaj.org/article/f5d5669ea35a43eb950b0c11af990ab1 |
| رقم الأكسشن: | edsdoj.f5d5669ea35a43eb950b0c11af990ab1 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0064561 |