دورية أكاديمية
The role of Nrf2 in astragaloside IV-mediated antioxidative protection on heart failure
| العنوان: | The role of Nrf2 in astragaloside IV-mediated antioxidative protection on heart failure |
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| المؤلفون: | Yan-Bo Sui, Kui-Kui Zhang, Yu-kun Ren, Li Liu, Yan Liu |
| المصدر: | Pharmaceutical Biology, Vol 58, Iss 1, Pp 1201-1207 (2020) |
| بيانات النشر: | Taylor & Francis Group, 2020. |
| سنة النشر: | 2020 |
| المجموعة: | LCC:Therapeutics. Pharmacology |
| مصطلحات موضوعية: | oxidative stress, ho-1, keap-1, transcriptional activation, Therapeutics. Pharmacology, RM1-950 |
| الوصف: | Context Heart failure is one of the most serious diseases worldwide. Astragaloside IV (ASI) is widely used in the treatment of cardiovascular diseases. Objective To elucidate the antioxidative mechanism of ASI in a rat model of left coronary artery ligation. Materials and methods Left coronary artery of Sprague–Dawley rats was ligated to establish the model of heart failure, and then vehicle (saline) or ASI (1 mg/kg/day) was orally administered to the rats (n = 15) for 6 weeks. Echocardiography was used to evaluate the cardiac function. Myocardial infarct size was measured by triphenyltetrazolium chloride staining. Oxidative stress in the ventricular myocardium was determined. Molecular mechanisms were investigated by Western blot and chromatin immunoprecipitation. Results ASI improved the cardiac function, especially ejection fraction (75.27 ± 5.75% vs. 36.26 ± 4.14%) and fractional shortening (45.39 ± 3.66% vs. 17.88 ± 1.32%), and reduced the infarct size of left ventricle (20.69 ± 2.98% vs. 39.11 ± 3.97%). ASI maintained the levels of glutathione, catalase and superoxide dismutase and prevented the leakage of creatine kinase. In addition, ASI induced the protein expression of Nrf2 (1.97-fold) and HO-1 (2.79-fold), while reduced that of Keap-1 (0.77-fold) in the ventricular myocardium. In H9c2 cells, a rat cardiomyocyte cell line, ASI induced the translocation of Nrf2 from cytoplasm to nucleus, followed by transcriptional activation of NQO-1 (8.27-fold), SOD-2 (3.27-fold) and Txn-1 (9.83-fold) genes. Discussion and conclusions ASI prevented heart failure by counteracting oxidative stress through the Nrf2/HO-1 pathway. Application in clinical practice warrants further investigation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1388-0209 1744-5116 13880209 |
| Relation: | https://doaj.org/toc/1388-0209; https://doaj.org/toc/1744-5116 |
| DOI: | 10.1080/13880209.2020.1849319 |
| URL الوصول: | https://doaj.org/article/f640f2adf07542d7b83338f010f5b2d2 |
| رقم الأكسشن: | edsdoj.f640f2adf07542d7b83338f010f5b2d2 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 13880209 17445116 |
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| DOI: | 10.1080/13880209.2020.1849319 |