دورية أكاديمية

lncRNA 5430416N02Rik Promotes the Proliferation of Mouse Embryonic Stem Cells by Activating Mid1 Expression through 3D Chromatin Architecture

التفاصيل البيبلوغرافية
العنوان: lncRNA 5430416N02Rik Promotes the Proliferation of Mouse Embryonic Stem Cells by Activating Mid1 Expression through 3D Chromatin Architecture
المؤلفون: Tong Zhao, Mingyang Cai, Man Liu, Guangsong Su, Daniel An, Byoungsan Moon, Guochang Lyu, Yibo Si, Lingyi Chen, Wange Lu
المصدر: Stem Cell Reports, Vol 14, Iss 3, Pp 493-505 (2020)
بيانات النشر: Elsevier, 2020.
سنة النشر: 2020
المجموعة: LCC:Medicine (General)
LCC:Biology (General)
مصطلحات موضوعية: Medicine (General), R5-920, Biology (General), QH301-705.5
الوصف: Summary: Both 3D chromatin architecture and long non-coding RNAs (lncRNAs) play essential roles in pluripotency maintenance. However, whether lncRNAs are involved in organizing 3D chromatin structure remains largely unexplored. We identified 39 lncRNAs bound by Klf4, among which we further revealed the 5430416N02Rik promoter is a chromatin interaction hub. Knockout of the 5430416N02Rik locus reduces the proliferation rate of embryonic stem cells (ESCs). Moreover, deleting both the promoter and the gene body of 5430416N02Rik causes a more severe proliferation defect and has a more profound impact on the transcriptome than deleting the gene body alone. The reduced proliferation of the 5430416N02Rik locus knockout ESCs is mainly due to the downregulation of Mid1, the expression of which requires the inter-chromosomal interaction between Mid1 and 5430416N02Rik loci. In summary, our data demonstrated that the lncRNA 5430416N02Rik gene locus maintains the fast proliferation of ESCs by activating the expression of Mid1 through chromatin interaction. : In this article, Chen and Lu and colleagues show that 5430416N02Rik, a long non-coding RNA (lncRNA) gene, is involved in organizing 3D chromatin structure in embryonic stem cells (ESCs). It is revealed that 5430416N02Rik gene serves as the chromatin interaction hub. In addition, 5430416N02Rik gene locus maintains the fast proliferation of ESCs by activating the expression of Mid1 through chromatin interaction. Keywords: 5430416N02Rik, lncRNA, 3D chromatin structure, embryonic stem cells
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2213-6711
Relation: http://www.sciencedirect.com/science/article/pii/S2213671120300588; https://doaj.org/toc/2213-6711
DOI: 10.1016/j.stemcr.2020.02.002
URL الوصول: https://doaj.org/article/f6500fadbfe7404ea4d25f2c0c86c811
رقم الأكسشن: edsdoj.f6500fadbfe7404ea4d25f2c0c86c811
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:22136711
DOI:10.1016/j.stemcr.2020.02.002