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Metabolite Analysis of Alternaria Mycotoxins by LC-MS/MS and Multiple Tools

التفاصيل البيبلوغرافية
العنوان: Metabolite Analysis of Alternaria Mycotoxins by LC-MS/MS and Multiple Tools
المؤلفون: Yanli You, Qinghua Hu, Nan Liu, Cuiju Xu, Sunan Lu, Tongcheng Xu, Xin Mao
المصدر: Molecules, Vol 28, Iss 7, p 3258 (2023)
بيانات النشر: MDPI AG, 2023.
سنة النشر: 2023
المجموعة: LCC:Organic chemistry
مصطلحات موضوعية: Alternaria, modified mycotoxins, metabolic pathway, molecular networking, feature-based molecular networking, Organic chemistry, QD241-441
الوصف: Alternaria fungi are widely distributed plant pathogens that invade crop products, causing significant economic damage. In addition, toxic secondary metabolites produced by the fungi can also endanger consumers. Many of these secondary metabolites are chemically characterized as mycotoxins. In this study, Q Exactive Orbitrap mass spectrometry was used for the non-targeted analysis of the metabolome of seven Alternaria isolates cultured on Potato Carrot Agar (PCA), Potato Dextrose Agar (PDA) and Potato Sucrose Agar (PSA) medium. Due to the difficulty of detecting modified toxins, an analytical strategy with multiple visual analysis tools was also used to determine the presence of sulfate conjugated toxins, as well as to visualize the molecular network of Alternaria toxins. The results show that PSA medium exhibits more advantageous properties for the culture of Alternaria, with more toxigenic species and quantities and more obvious metabolic pathways. Based on high-resolution tandem mass spectrometry (MS/MS) data, the mycotoxins and their metabolites were mainly clustered into four groups: alternariol (AOH)/alternariol monomethyl ether (AME)/altenusin (ALU)/altenuene (ALT)/dehydroaltenusin (DHA)/Desmethyldehydroaltenusin (DMDA) families, Altertoxin-I (ATX-I) family, tentoxin (TEN) family and tenuazonic acid (TeA) family. Moreover, the PSA medium is more suitable for the accumulation of AOH, AME, ALU, ALT, DHA and DMDA, while the PDA medium is more suitable for the accumulation of ATX-I, TEN and TeA. This research may provide theoretical support for the metabolomics study of Alternaria.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 28073258
1420-3049
Relation: https://www.mdpi.com/1420-3049/28/7/3258; https://doaj.org/toc/1420-3049
DOI: 10.3390/molecules28073258
URL الوصول: https://doaj.org/article/f6c211bd75354f448f6d6869738aad3b
رقم الأكسشن: edsdoj.f6c211bd75354f448f6d6869738aad3b
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:28073258
14203049
DOI:10.3390/molecules28073258