دورية أكاديمية

Inhibition of calpain9 attenuates peritoneal dialysis-related peritoneal fibrosis

التفاصيل البيبلوغرافية
العنوان: Inhibition of calpain9 attenuates peritoneal dialysis-related peritoneal fibrosis
المؤلفون: Fang Li, Yu Wang, Jianwei Tian, Zhanmei Zhou, Wei Yin, Xianhui Qin, Huizhen Wang, Tao Zeng, Aiqing Li, Jianping Jiang
المصدر: Frontiers in Pharmacology, Vol 13 (2022)
بيانات النشر: Frontiers Media S.A., 2022.
سنة النشر: 2022
المجموعة: LCC:Therapeutics. Pharmacology
مصطلحات موضوعية: calpain, calpain 9, fibrosis, peritoneal dialysis, peritoneal mesothelial cells, Therapeutics. Pharmacology, RM1-950
الوصف: Aim: Peritoneal dialysis is a common renal replacement method for end-stage renal disease. Long-term peritoneal dialysis leads to peritoneal dialysis-related peritoneal fibrosis, which leads to a cessation of treatment. Calpain is a protein belonging to calcium-dependent endopeptidase family and plays an important role in extracellular matrix remodeling. Here, we evaluated the effect of calpain in peritoneal dialysis-related peritoneal fibrosis.Methods: We established two animal models of peritoneal fibrosis and inhibited the activity of Calpain, and then collected peritoneal tissue to evaluate the progress of fibrosis and the changes of Calpain and β-catenin. We obtained Rat peritoneal mesothelial cells and Human peritoneal mesothelial cell line and stimulated with TGF-β to produce extracellular matrix. Next we inhibited Calpain activity or reduced Calpain9 expression, and then assessed changes in extracellular matrix and β-catenin.Results: Inhibition of calpain activity attenuated chlorhexidine glucose and peritoneal dialysis-induced peritoneal thickening and β-catenin expression in mice. In addition, compared with the control group, when primary rat peritoneal mesothelial cells or human peritoneal mesothelial cells were treated with transforming growth factor beta, down-regulation of calpain activity inhibited the expression of Fibronectin and Collagen I, and increased the expression of E-cadherin. These changes could be adjusted after silencing calpain9. Finally, calpain9 deficiency was associated with down-regulation of Fibronectin and β-catenin in human peritoneal mesothelial cells.Conclusion: Our results suggest that calpain9 may be a key molecule in mediating peritoneal dialysis-related peritoneal fibrosis.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1663-9812
Relation: https://www.frontiersin.org/articles/10.3389/fphar.2022.962770/full; https://doaj.org/toc/1663-9812
DOI: 10.3389/fphar.2022.962770
URL الوصول: https://doaj.org/article/cf77e1b38f614b9397860904329b9477
رقم الأكسشن: edsdoj.f77e1b38f614b9397860904329b9477
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:16639812
DOI:10.3389/fphar.2022.962770