دورية أكاديمية
Development of an antibody against EtpA from enterotoxigenic Escherichia coli and evaluation of its use for bacterial isolation using magnetic beads
| العنوان: | Development of an antibody against EtpA from enterotoxigenic Escherichia coli and evaluation of its use for bacterial isolation using magnetic beads |
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| المؤلفون: | HELBER A. ASTOLPHO, LUIS ANDRÉ M. MARIÚBA, YURY O. CHAVES, JULIANE C. GLÓRIA, MARIA EDILENE M. DE ALMEIDA, RICARDO A. MACHADO-DE-ÁVILA, CAMILLE M. GENEROSO, JENIFFER C.L. BATISTA, PAULO AFONSO NOGUEIRA, PATRÍCIA P. ORLANDI |
| المصدر: | Anais da Academia Brasileira de Ciências, Vol 96, Iss 2 (2024) |
| بيانات النشر: | Academia Brasileira de Ciências, 2024. |
| سنة النشر: | 2024 |
| المجموعة: | LCC:Science |
| مصطلحات موضوعية: | diarrheagenic, detection, ETEC, magnetic beads, Science |
| الوصف: | Abstract The enterotoxigenic Escherichia coli (ETEC) strain is one of the most frequent causative agents of childhood diarrhea and travelers’ diarrhea in low-and middle-income countries. Among the virulence factors secreted by ETEC, the exoprotein EtpA has been described as an important. In the present study, a new detection tool for enterotoxigenic E. coli bacteria using the EtpA protein was developed. Initially, antigenic sequences of the EtpA protein were selected via in silico prediction. A chimeric recombinant protein, corresponding to the selected regions, was expressed in an E. coli host, purified and used for the immunization of mice. The specific recognition of anti-EtpA IgG antibodies generated was evaluated using flow cytometry. The tests demonstrated that the antibodiesdeveloped were able to recognize the native EtpA protein. By coupling these antibodies to magnetic beads for the capture and detection of ETEC isolates, cytometric analyses showed an increase in sensitivity, specificity and the effectiveness of the method of separation and detection of these pathogens. This is the first report of the use of this methodology for ETEC separation. Future trials may indicate their potential use for isolating these and other pathogens in clinical samples, thus accelerating the diagnosis and treatment of diseases. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1678-2690 0001-3765 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652024000200802&lng=en&tlng=en; http://www.scielo.br/pdf/aabc/v96n2/0001-3765-aabc-96-02-e20231208.pdf; https://doaj.org/toc/1678-2690 |
| DOI: | 10.1590/0001-3765202420231208 |
| URL الوصول: | https://doaj.org/article/f86db8cd30d64f6d8738db3268b342b9 |
| رقم الأكسشن: | edsdoj.f86db8cd30d64f6d8738db3268b342b9 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 16782690 00013765 |
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| DOI: | 10.1590/0001-3765202420231208 |