دورية أكاديمية
Treponema pallidum genome sequencing from six continents reveals variability in vaccine candidate genes and dominance of Nichols clade strains in Madagascar.
| العنوان: | Treponema pallidum genome sequencing from six continents reveals variability in vaccine candidate genes and dominance of Nichols clade strains in Madagascar. |
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| المؤلفون: | Nicole A P Lieberman, Michelle J Lin, Hong Xie, Lasata Shrestha, Tien Nguyen, Meei-Li Huang, Austin M Haynes, Emily Romeis, Qian-Qiu Wang, Rui-Li Zhang, Cai-Xia Kou, Giulia Ciccarese, Ivano Dal Conte, Marco Cusini, Francesco Drago, Shu-Ichi Nakayama, Kenichi Lee, Makoto Ohnishi, Kelika A Konda, Silver K Vargas, Maria Eguiluz, Carlos F Caceres, Jeffrey D Klausner, Oriol Mitjà, Anne Rompalo, Fiona Mulcahy, Edward W Hook, Sheila A Lukehart, Amanda M Casto, Pavitra Roychoudhury, Frank DiMaio, Lorenzo Giacani, Alexander L Greninger |
| المصدر: | PLoS Neglected Tropical Diseases, Vol 15, Iss 12, p e0010063 (2021) |
| بيانات النشر: | Public Library of Science (PLoS), 2021. |
| سنة النشر: | 2021 |
| المجموعة: | LCC:Arctic medicine. Tropical medicine LCC:Public aspects of medicine |
| مصطلحات موضوعية: | Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270 |
| الوصف: | In spite of its immutable susceptibility to penicillin, Treponema pallidum (T. pallidum) subsp. pallidum continues to cause millions of cases of syphilis each year worldwide, resulting in significant morbidity and mortality and underscoring the urgency of developing an effective vaccine to curtail the spread of the infection. Several technical challenges, including absence of an in vitro culture system until very recently, have hampered efforts to catalog the diversity of strains collected worldwide. Here, we provide near-complete genomes from 196 T. pallidum strains-including 191 T. pallidum subsp. pallidum-sequenced directly from patient samples collected from 8 countries and 6 continents. Maximum likelihood phylogeny revealed that samples from most sites were predominantly SS14 clade. However, 99% (84/85) of the samples from Madagascar formed two of the five distinct Nichols subclades. Although recombination was uncommon in the evolution of modern circulating strains, we found multiple putative recombination events between T. pallidum subsp. pallidum and subsp. endemicum, shaping the genomes of several subclades. Temporal analysis dated the most recent common ancestor of Nichols and SS14 clades to 1717 (95% HPD: 1543-1869), in agreement with other recent studies. Rates of SNP accumulation varied significantly among subclades, particularly among different Nichols subclades, and was associated in the Nichols A subclade with a C394F substitution in TP0380, a ERCC3-like DNA repair helicase. Our data highlight the role played by variation in genes encoding putative surface-exposed outer membrane proteins in defining separate lineages, and provide a critical resource for the design of broadly protective syphilis vaccines targeting surface antigens. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1935-2727 1935-2735 |
| Relation: | https://doaj.org/toc/1935-2727; https://doaj.org/toc/1935-2735 |
| DOI: | 10.1371/journal.pntd.0010063 |
| URL الوصول: | https://doaj.org/article/f8f88374a8944331bc4a55f2256ab665 |
| رقم الأكسشن: | edsdoj.f8f88374a8944331bc4a55f2256ab665 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19352727 19352735 |
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| DOI: | 10.1371/journal.pntd.0010063 |