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Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker.

التفاصيل البيبلوغرافية
العنوان: Protoplast-mediated transformation of Madurella mycetomatis using hygromycin resistance as a selection marker.
المؤلفون: Saskia du Pré, Mickey Konings, Dorenda J A Schoorl, Ahmed H Fahal, Mark Arentshorst, Arthur F J Ram, Wendy W J van de Sande
المصدر: PLoS Neglected Tropical Diseases, Vol 18, Iss 4, p e0012092 (2024)
بيانات النشر: Public Library of Science (PLoS), 2024.
سنة النشر: 2024
المجموعة: LCC:Arctic medicine. Tropical medicine
LCC:Public aspects of medicine
مصطلحات موضوعية: Arctic medicine. Tropical medicine, RC955-962, Public aspects of medicine, RA1-1270
الوصف: Madurella mycetomatis is the main cause of mycetoma, a chronic granulomatous infection for which currently no adequate therapy is available. To improve therapy, more knowledge on a molecular level is required to understand how M. mycetomatis is able to cause this disease. However, the genetic toolbox for M. mycetomatis is limited. To date, no method is available to genetically modify M. mycetomatis. In this paper, a protoplast-mediated transformation protocol was successfully developed for this fungal species, using hygromycin as a selection marker. Furthermore, using this method, a cytoplasmic-GFP-expressing M. mycetomatis strain was created. The reported methodology will be invaluable to explore the pathogenicity of M. mycetomatis and to develop reporter strains which can be useful in drug discovery as well as in genetic studies.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1935-2727
1935-2735
Relation: https://journals.plos.org/plosntds/article/file?id=10.1371/journal.pntd.0012092&type=printable; https://doaj.org/toc/1935-2727; https://doaj.org/toc/1935-2735
DOI: 10.1371/journal.pntd.0012092&type=printable
DOI: 10.1371/journal.pntd.0012092
URL الوصول: https://doaj.org/article/f947d9254d8c4d988d78b36597ff967c
رقم الأكسشن: edsdoj.f947d9254d8c4d988d78b36597ff967c
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:19352727
19352735
DOI:10.1371/journal.pntd.0012092&type=printable