دورية أكاديمية
Phenotypic diversity and selection maintain Leishmania amazonensis infectivity in BALB/c mouse model
| العنوان: | Phenotypic diversity and selection maintain Leishmania amazonensis infectivity in BALB/c mouse model |
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| المؤلفون: | Benoît Espiau, Virginia Vilhena, Armelle Cuvillier, Aldina Barral, Gilles Merlin |
| المصدر: | Memorias do Instituto Oswaldo Cruz, Vol 112, Iss 1, Pp 44-52 |
| بيانات النشر: | Fundação Oswaldo Cruz (FIOCRUZ). |
| المجموعة: | LCC:Microbiology LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Leishmania amazonensis, subcloning, phenotypic diversity, infectivity, selection, Microbiology, QR1-502, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Leishmania are protozoan parasites that show remarkable diversity, as revealed by the various clinical forms of leishmaniasis, which can range from mild skin lesions to severe metastatic cutaneous/mucosal lesions. The exact nature and extent of Leishmania phenotypic diversity in establishing infection is not fully understood. In order to try to understand some aspects of this diversity, we subcutaneously infected BALB/c mice with first and second generation subclones of a L. amazonensis strain isolated from a patient (BA125) and examined in vivo lesion growth rate and antimony susceptibility. In vivo fast-, medium- and slow-growing subclones were obtained; moreover, fast-growing subclones could generate slow-growing subclones and inversely, revealing the continuous generation of diversity after passage into mice. No antimony-resistant subclone appeared, probably a rare occurrence. By tagging subclone cells with a L. amazonensis genomic cosmid library, we found that only a very small number of founding cells could produce lesions. Leishmania clones transfected with in vivo selected individual cosmids were also diverse in terms of lesion growth rate, revealing the cosmid-independent intrinsic characteristics of each clone. Our results suggest that only a few of the infecting parasites are able to grow and produce lesions; later, within the cell mixture of each lesion, there coexist several parasite populations with different potentialities to grow lesions during the next infection round. This may reflect a sort of programmed heterogeneity of individual parasites, favoring the survival of some individuals in various environmental conditions. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1678-8060 0074-0276 |
| Relation: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762017000100044&lng=en&tlng=en; https://doaj.org/toc/1678-8060 |
| DOI: | 10.1590/0074-02760160280 |
| URL الوصول: | https://doaj.org/article/f9886d79d1674c72ac548caad4ba431f |
| رقم الأكسشن: | edsdoj.f9886d79d1674c72ac548caad4ba431f |
| قاعدة البيانات: | Directory of Open Access Journals |
| تدمد: | 16788060 00740276 |
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| DOI: | 10.1590/0074-02760160280 |