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Discrimination and Quantification of Glutathione by Cu+-Based Nanozymes

التفاصيل البيبلوغرافية
العنوان: Discrimination and Quantification of Glutathione by Cu+-Based Nanozymes
المؤلفون: Meixuan Liu, Chen Yan, Qianyun Ye, Xiaohuan Sun, Jie Han
المصدر: Biosensors, Vol 13, Iss 8, p 827 (2023)
بيانات النشر: MDPI AG, 2023.
سنة النشر: 2023
المجموعة: LCC:Biotechnology
مصطلحات موضوعية: nanozyme, GSH quantification, GSH discrimination, Biotechnology, TP248.13-248.65
الوصف: Glutathione (GSH) is the most abundant low-molecular-weight biological thiol in vivo and has been linked to several diseases. The accurate quantification of GSH is therefore crucial for disease diagnosis and monitoring. In this study, we prepared self-assembled Cu(I)-Cys (cysteine) nanozymes through a two-step procedure. The Cu(I)-Cys nanoparticles exhibited peroxidase-mimicking activity. Upon the addition of H2O2, they were able to oxidize 3,3,5,5-tetramethylbenzidine (TMB) into oxTMB, resulting in a measurable increase in UV-Vis absorption at 655 nm. However, in the presence of GSH, oxTMB was reduced back to TMB, leading to a decrease in UV-Vis absorption at 655 nm. By utilizing these changes in the absorption intensity, we achieved the sensitive detection of GSH with a detection limit of 2.13 μM. Moreover, taking advantage of the different peroxidase-mimicking activities of Cu(I)-Cys nanoparticles at various pH values, a sensor array with Cu(I)-Cys nanoparticles at pH 4 and pH 5 was constructed. The discrimination of GSH among Cys and ascorbic acid was achieved and the practicability of the sensor array in human serum was validated. This novel approach holds significant promise for the precise discrimination and quantification of GSH and its potential applications in disease diagnosis and therapeutics.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 2079-6374
07201745
Relation: https://www.mdpi.com/2079-6374/13/8/827; https://doaj.org/toc/2079-6374
DOI: 10.3390/bios13080827
URL الوصول: https://doaj.org/article/f996f18db1a64dfe8000cc07201745dc
رقم الأكسشن: edsdoj.f996f18db1a64dfe8000cc07201745dc
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:20796374
07201745
DOI:10.3390/bios13080827