دورية أكاديمية
Depletion of regulatory T-cells leads to moderate B-cell antigenicity in respiratory syncytial virus infection
| العنوان: | Depletion of regulatory T-cells leads to moderate B-cell antigenicity in respiratory syncytial virus infection |
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| المؤلفون: | Hsiao-Yun Shao, Juo-Yu Huang, Yi-Wen Lin, Shu-Ling Yu, Ebenezer Chitra, Ching-Kun Chang, Wang-Chou Sung, Pele Chong, Yen-Hung Chow |
| المصدر: | International Journal of Infectious Diseases, Vol 41, Iss C, Pp 56-64 (2015) |
| بيانات النشر: | Elsevier, 2015. |
| سنة النشر: | 2015 |
| المجموعة: | LCC:Infectious and parasitic diseases |
| مصطلحات موضوعية: | Respiratory syncytial virus, B-cells, Antigenicity, Regulatory T-cells, Infectious and parasitic diseases, RC109-216 |
| الوصف: | Objectives: The regulation of the immunopathology of respiratory syncytial virus (RSV) by regulatory T-cells (CD4+CD25+Foxp3+; Tregs) is not understood. Methods: To deduce the same, Tregs were depleted in BALB/c mice by injecting anti-CD25 antibody followed by RSV infection (anti-CD25-RSV mice). Results: In this model, a decrease in anti-fusion (F) antibody and neutralizing activity, and an increase in anti-nucleocapsid (N) antibody in serum, were seen. Decreased antibody-dependent cell-mediated cytotoxicity (ADCC) activity, increased IgG2a, and an influx of activated CD8+ T-cells into the lungs were also observed. Co-culture of splenic CD45RA+ B-cells from RSV-infected normal mice with CD4+ cells isolated from anti-CD25-RSV mice (B/CD4) increased anti-F antibody secretion. The inclusion of CD25+ Tregs isolated from isotype Ig-RSV mice into the B/CD4 co-culture substantially enhanced the frequency of anti-F antibody production. However, the same effect was not seen in the co-culture of CD45RA+ B-cells with dendritic cells (DCs) (B/DCs) or CD8+ cells (B/CD8) that were obtained from anti-CD25-RSV mice. The transfer of enriched B-cells from anti-CD25-RSV mice into RSV-infected SCID mice increased severe lung inflammation associated with the increased viral load and eosinophil number. Conclusions: These results indicate that Tregs modulate B-cell activity, particularly in producing F-specific neutralizing antibodies, to regulate RSV-mediated exacerbated diseases. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1201-9712 1878-3511 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S120197121500260X; https://doaj.org/toc/1201-9712; https://doaj.org/toc/1878-3511 |
| DOI: | 10.1016/j.ijid.2015.10.026 |
| URL الوصول: | https://doaj.org/article/f9b2d845cfc2494aaf1e0e9a5a3ce482 |
| رقم الأكسشن: | edsdoj.f9b2d845cfc2494aaf1e0e9a5a3ce482 |
| قاعدة البيانات: | Directory of Open Access Journals |
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Full Text Finder | تدمد: | 12019712 18783511 |
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| DOI: | 10.1016/j.ijid.2015.10.026 |