دورية أكاديمية
Preventive effects of the butanol fraction of Justicia procumbens L. against dexamethasone-induced muscle atrophy in C2C12 myotubes
| العنوان: | Preventive effects of the butanol fraction of Justicia procumbens L. against dexamethasone-induced muscle atrophy in C2C12 myotubes |
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| المؤلفون: | Jae-Yong Kim, Hye Mi Kim, Ji Hoon Kim, Ju-Hee Lee, Kaixuan Zhang, Shuo Guo, Do Hyun Lee, Eun Mei Gao, Rak Ho Son, Seong-Min Kim, Chul Young Kim |
| المصدر: | Heliyon, Vol 8, Iss 11, Pp e11597- (2022) |
| بيانات النشر: | Elsevier, 2022. |
| سنة النشر: | 2022 |
| المجموعة: | LCC:Science (General) LCC:Social sciences (General) |
| مصطلحات موضوعية: | Justicia procumbens L., Muscle atrophy, C2C12, Dexamethasone, Protein degradation, Science (General), Q1-390, Social sciences (General), H1-99 |
| الوصف: | Skeletal muscle atrophy is associated with many diseases including cancer, inflammatory diseases, neuromuscular diseases, and acute critical illness. Justicia procumbens L. has been used as a herbal remedy, but the pharmacological effect of J. procumbens on muscle atrophy has not yet been reported. Herein, we investigate the anti-atrophic effect of the n-butanol fraction of J. procumbens (JPBuFr) on dexamethasone (DEX)-induced muscle atrophy in C2C12 myotubes. The myotubes diameter, MHC positive area, ROS production, and mitochondria contents were observed under a fluorescence microscope, and various proteins related to degradation or synthesis were analyzed by western blots. JPBuFr significantly attenuated a reduction of myotube diameter, mitochondrial content, ATP level, myosin heavy chain, and myogenin expression induced by DEX. Furthermore, co-treatment of DEX and JPBuFr not only increased phosphorylation of Akt, mTOR, and p70S6K proteins but also decreased reactive oxygen species production and expression of protein degradation factors (MuRF1, Atrogin-1, FoxO3a) compared to DEX treatment. These results suggest that JPBuFr may provide potential protective effects against muscle atrophy, giving it potential for the development of anti-atrophic health functional foods. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2405-8440 |
| Relation: | http://www.sciencedirect.com/science/article/pii/S2405844022028857; https://doaj.org/toc/2405-8440 |
| DOI: | 10.1016/j.heliyon.2022.e11597 |
| URL الوصول: | https://doaj.org/article/f9dc7692c48e4aacbd713b9196c8ea13 |
| رقم الأكسشن: | edsdoj.f9dc7692c48e4aacbd713b9196c8ea13 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 24058440 |
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| DOI: | 10.1016/j.heliyon.2022.e11597 |