دورية أكاديمية
Invasions of gladiolus rust in North America are caused by a widely-distributed clone of Uromycestransversalis
| العنوان: | Invasions of gladiolus rust in North America are caused by a widely-distributed clone of Uromycestransversalis |
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| المؤلفون: | Jeffery A. DeLong, Jane E. Stewart, Alberto Valencia-Botín, Kerry F. Pedley, James W. Buck, Marin T. Brewer |
| المصدر: | PeerJ, Vol 7, p e7986 (2019) |
| بيانات النشر: | PeerJ Inc., 2019. |
| سنة النشر: | 2019 |
| المجموعة: | LCC:Medicine LCC:Biology (General) |
| مصطلحات موضوعية: | Clonal population, Invasion, Ornamental rust, Whole genome sequencing, Genetic diversity, Microsatellites, Medicine, Biology (General), QH301-705.5 |
| الوصف: | Uromyces transversalis, the causal agent of Gladiolus rust, is an invasive plant pathogen in the United States and is regulated as a quarantine pathogen in Europe. The aim of this research was to: (i) determine the origin of introductions of U. transversalis to the United States, (ii) track the movement of genotypes, and (iii) understand the worldwide genetic diversity of the species. To develop molecular markers for genotyping, whole genome sequencing was performed on three isolates collected in the United States. Genomes were assembled de novo and searched for microsatellite regions. Primers were developed and tested on ten isolates from the United States resulting in the identification of 24 polymorphic markers. Among 92 isolates collected from Costa Rica, Mexico, New Zealand, Australia, and the United States there were polymorphisms within isolates with no genotypic diversity detected among isolates; however, missing data among the New Zealand and Australia isolates due to either poor amplification of degraded DNA or null alleles as a result of genetic differences made it difficult to generate conclusions about these populations. The microsatellite loci and flanking regions showed high diversity and two divergent genomes within dikaryotic individuals, yet no diversity among individuals, suggesting that the invasive U. transversalis populations from North America are strictly clonal. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 2167-8359 |
| Relation: | https://peerj.com/articles/7986.pdf; https://peerj.com/articles/7986/; https://doaj.org/toc/2167-8359 |
| DOI: | 10.7717/peerj.7986 |
| URL الوصول: | https://doaj.org/article/df9fb68579b9455aa82b1e4387a32a85 |
| رقم الأكسشن: | edsdoj.f9fb68579b9455aa82b1e4387a32a85 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 21678359 |
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| DOI: | 10.7717/peerj.7986 |