دورية أكاديمية
Novel microRNA candidates and miRNA-mRNA pairs in embryonic stem (ES) cells.
| العنوان: | Novel microRNA candidates and miRNA-mRNA pairs in embryonic stem (ES) cells. |
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| المؤلفون: | Peili Gu, Jeffrey G Reid, Xiaolian Gao, Chad A Shaw, Chad Creighton, Peter L Tran, Xiaochuan Zhou, Rafal B Drabek, David L Steffen, David M Hoang, Michelle K Weiss, Arash O Naghavi, Jad El-daye, Mahjabeen F Khan, Glen B Legge, David A Wheeler, Richard A Gibbs, Jonathan N Miller, Austin J Cooney, Preethi H Gunaratne |
| المصدر: | PLoS ONE, Vol 3, Iss 7, p e2548 (2008) |
| بيانات النشر: | Public Library of Science (PLoS), 2008. |
| سنة النشر: | 2008 |
| المجموعة: | LCC:Medicine LCC:Science |
| مصطلحات موضوعية: | Medicine, Science |
| الوصف: | MicroRNAS (miRNAS: a class of short non-coding RNAs) are emerging as important agents of post transcriptional gene regulation and integral components of gene networks. MiRNAs have been strongly linked to stem cells, which have a remarkable dual role in development. They can either continuously replenish themselves (self-renewal), or differentiate into cells that execute a limited number of specific actions (pluripotence).In order to identify novel miRNAs from narrow windows of development we carried out an in silico search for micro-conserved elements (MCE) in adult tissue progenitor transcript sequences. A plethora of previously unknown miRNA candidates were revealed including 545 small RNAs that are enriched in embryonic stem (ES) cells over adult cells. Approximately 20% of these novel candidates are down-regulated in ES (Dicer(-/-)) ES cells that are impaired in miRNA maturation. The ES-enriched miRNA candidates exhibit distinct and opposite expression trends from mmu-mirs (an abundant class in adult tissues) during retinoic acid (RA)-induced ES cell differentiation. Significant perturbation of trends is found in both miRNAs and novel candidates in ES (GCNF(-/-)) cells, which display loss of repression of pluripotence genes upon differentiation.Combining expression profile information with miRNA target prediction, we identified miRNA-mRNA pairs that correlate with ES cell pluripotence and differentiation. Perturbation of these pairs in the ES (GCNF(-/-)) mutant suggests a role for miRNAs in the core regulatory networks underlying ES cell self-renewal, pluripotence and differentiation. |
| نوع الوثيقة: | article |
| وصف الملف: | electronic resource |
| اللغة: | English |
| تدمد: | 1932-6203 |
| Relation: | http://europepmc.org/articles/PMC2481296?pdf=render; https://doaj.org/toc/1932-6203 |
| DOI: | 10.1371/journal.pone.0002548 |
| URL الوصول: | https://doaj.org/article/fa0e98b63c60468398a4c6e40e88f2e7 |
| رقم الأكسشن: | edsdoj.fa0e98b63c60468398a4c6e40e88f2e7 |
| قاعدة البيانات: | Directory of Open Access Journals |
Full Text Finder | تدمد: | 19326203 |
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| DOI: | 10.1371/journal.pone.0002548 |