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Long noncoding RNA H19 regulates the therapeutic efficacy of mesenchymal stem cells in rats with severe acute pancreatitis by sponging miR-138-5p and miR-141-3p

التفاصيل البيبلوغرافية
العنوان: Long noncoding RNA H19 regulates the therapeutic efficacy of mesenchymal stem cells in rats with severe acute pancreatitis by sponging miR-138-5p and miR-141-3p
المؤلفون: Guodong Song, Jia Zhou, Ruimei Song, Dalu Liu, Weidi Yu, Wangcheng Xie, Zhilong Ma, Jian Gong, Hongbo Meng, Tingsong Yang, Zhenshun Song
المصدر: Stem Cell Research & Therapy, Vol 11, Iss 1, Pp 1-15 (2020)
بيانات النشر: BMC, 2020.
سنة النشر: 2020
المجموعة: LCC:Medicine (General)
LCC:Biochemistry
مصطلحات موضوعية: Long noncoding RNA H19, Mesenchymal stem cells, Severe acute pancreatitis, Autophagy, Cell proliferation, Medicine (General), R5-920, Biochemistry, QD415-436
الوصف: Abstract Background Patients with severe acute pancreatitis (SAP), which is characterized by high morbidity and mortality, account for an increasing medical burden worldwide. We previously found that mesenchymal stem cells (MSCs) could attenuate SAP and that expression of long noncoding RNA H19 (LncRNA H19) was upregulated in rats receiving MSCs. In the present study, we investigated the mechanisms of LncRNA H19 regulating the therapeutic efficacy of MSCs in the alleviation of SAP. Methods MSCs transfected with LncRNA H19 overexpression and knockdown plasmids were intravenously injected into rats 12 h after sodium taurocholate (NaT) administration to induce SAP. Results Overexpressing LncRNA H19 in MSCs significantly enhanced the anti-inflammatory capacity of the MSCs, inhibited autophagy via promotion of focal adhesion kinase (FAK)-associated pathways, and facilitated cell proliferation by increasing the level of β-catenin in rats with SAP. LncRNA H19 functioned as a competing endogenous RNA by sponging miR-138-5p and miR-141-3p. Knocking down miR-138-5p in MSCs increased the expression of protein tyrosine kinase 2 (PTK2, encoding FAK) to suppress autophagy, while downregulating miR-141-3p enhanced the level of β-catenin to promote cell proliferation. Conclusions In conclusion, LncRNA H19 effectively increased the therapeutic efficacy of MSCs in rats with SAP via the miR-138-5p/PTK2/FAK and miR-141-3p/β-catenin pathways.
نوع الوثيقة: article
وصف الملف: electronic resource
اللغة: English
تدمد: 1757-6512
Relation: http://link.springer.com/article/10.1186/s13287-020-01940-z; https://doaj.org/toc/1757-6512
DOI: 10.1186/s13287-020-01940-z
URL الوصول: https://doaj.org/article/fa62e7ee2d844241828af4684b476524
رقم الأكسشن: edsdoj.fa62e7ee2d844241828af4684b476524
قاعدة البيانات: Directory of Open Access Journals
الوصف
تدمد:17576512
DOI:10.1186/s13287-020-01940-z